DNA methylation status of MutS genes in ameloblastoma

Autor(es)

do Amaral-Silva, Gleyson Kleber ; Fontes Pereira, Thais dos Santos ; Rocha, Andre Caroli ; Linhares Almeida Mariz, Bruno Augusto ; Prado-Ribeiro, Ana Carolina ; Fonseca, Felipe Paiva ; Gomez, Ricardo Santiago ; Vargas, Pablo Agustin

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 28, n. 7, p. 6-pg., 2021-04-30.

Resumo

Objectives Ameloblastoma is an odontogenic epithelial tumour with a low expression of mismatch repair system components. We aimed to investigate the methylation status of the genes MSH2, MSH3 and MSH6 (MutS group) in conventional ameloblastomas. Materials and Methods The ameloblastoma and dental follicle samples (n = 10 each) were collected from 20 different patients. Each ameloblastoma sample was sectioned into two fragments: one was paraffin-embedded while the other one, likewise the dental follicle samples, was fixed in RNAlater and frozen at -196 degrees C. All frozen samples were investigated for the MutS genes methylation levels, using the enzymatic restriction digestion and quantitative real-time PCR (qPCR) assay. The ameloblastoma paraffin-embedded samples were submitted to immunohistochemical reactions for MutS proteins detection and digitally quantification. Correlation analyses were performed between the immunohistochemical results and the respective gene methylation percentage. Results There are no significant differences between the MutS genes methylation levels in the ameloblastoma and the dental follicle. However, a strong negative correlation was found between MSH2 and MSH6 gene methylation status and their respective proteins expressions evaluated by immunohistochemistry. Conclusion Our results show that the genes methylations is in part responsible for decreasing the expression of MSH2 and MSH6 genes in ameloblastoma. (AU)

• Processo FAPESP: 17/08995-2 - Eventos epigenéticos em ameloblastomas: imunoexpressão de DNMT1, DNMT3b e H3K9ac e perfil de metilação de promotores hMutS
• Beneficiário: Gleyson Kleber do Amaral Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado