| Texto completo | |
| Autor(es): |
Tomazini, Atilio
;
Carvalho, Mariele
;
Murakami, Mario T. T.
;
Viviani, Vadim R.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. N/A, p. 12-pg., 2023-01-21. |
| Resumo | |
Beetle luciferases were classified into three functional groups: (1) pH-sensitive yellow-green-emitting (fireflies) which change the bioluminescence color to red at acidic pH, high temperatures and presence of heavy metals; (2) the pH-insensitive green-yellow-emitting (click beetles, railroad worms and firefly isozymes) which are not affected by these factors, and (3) pH-insensitive red-emitting. Although the pH-sensing site in firefly luciferases was recently identified, it is unclear why some luciferases are pH-insensitive despite the presence of some conserved pH-sensing residues. Through circular dichroism, we compared the secondary structural changes and unfolding temperature of luciferases of representatives of these three groups: (1) pH-sensitive green-yellow-emitting Macrolampis sp2 (Mac) and Amydetes vivianii (Amy) firefly luciferases; (2) the pH-insensitive green-emitting Pyrearinus termitilluminans larval click beetle (Pte) and Aspisoma lineatum (Al2) larval firefly luciferases, and (3) the pH-insensitive red-emitting Phrixotrix hirtus railroadworm (PxRE) luciferase. The most blue-shifted luciferases, independently of pH sensitivity, are thermally more stable at different pHs than the red-shifted ones. The pH-sensitive luciferases undergo increases of alpha-helices and thermal stability above pH 6. The pH-insensitive Pte luciferase secondary structure remains stable between pH 6 and 8, whereas the Al2 luciferase displays an increase of the beta-sheet at pH 8. The PxRE luciferase also displays an increase of alpha-helices at pH 8. The results indicate that green-yellow emission in beetle luciferases can be attained by: (1) a structurally rigid scaffold which stabilizes a single closed active site conformation in the pH-insensitive luciferases, and (2) active site compaction above pH 7.0 in the more flexible pH-sensitive luciferases. (AU) | |
| Processo FAPESP: | 10/05426-8 - Bioluminescência de artrópodes: diversidade biológica em biomas brasileiros; origem bioquímica; evolução estrutural/funcional de luciferases; diferenciação molecular das lanternas; aplicações biotecnológicas, ambientais e educacionais |
| Beneficiário: | Vadim Viviani |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 18/02538-1 - Resolução das estruturas tridimensionais de luciferases de besouros e enzimas relacionadas por cristalografia de raios X: relação entre estrutura, espectros de bioluminescência e atividade oxigenásica |
| Beneficiário: | Atílio Tomazini Júnior |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |