High-Resolution Magic-Angle-Spinning NMR in Revealing Hepatoblastoma Hallmarks

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Tasic, Ljubica ; Avramovic, Natasa ; Jadranin, Milka ; Quintero, Melissa ; Stanisic, Danijela ; Martins, Lucas G. G. ; Costa, Tassia Brena Barroso Carneiro ; Novak, Estela ; Odone, Vicente ; Rivas, Maria ; Aguiar, Talita ; Carraro, Dirce Maria ; da Cunha, Isabela Werneck ; da Costa, Cecilia Maria Lima ; Maschietto, Mariana ; Krepischi, Ana

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICINES; v. 10, n. 12, p. 11-pg., 2022-12-01.

Resumo

Cancer is one of the leading causes of death in children and adolescents worldwide; among the types of liver cancer, hepatoblastoma (HBL) is the most common in childhood. Although it affects only two to three individuals in a million, it is mostly asymptomatic at diagnosis, so by the time it is detected it has already advanced. There are specific recommendations regarding HBL treatment, and ongoing studies to stratify the risks of HBL, understand the pathology, and predict prognostics and survival rates. Although magnetic resonance imaging spectroscopy is frequently used in diagnostics of HBL, high-resolution magic-angle-spinning (HR-MAS) NMR spectroscopy of HBL tissues is scarce. Using this technique, we studied the alterations among tissue metabolites of ex vivo samples from (a) HBL and non-cancer liver tissues (NCL), (b) HBL and adjacent non-tumor samples, and (c) two regions of the same HBL samples, one more centralized and the other at the edge of the tumor. It was possible to identify metabolites in HBL, then metabolites from the HBL center and the border samples, and link them to altered metabolisms in tumor tissues, highlighting their potential as biochemical markers. Metabolites closely related to liver metabolisms such as some phospholipids, triacylglycerides, fatty acids, glucose, and amino acids showed differences between the tissues. (AU)

• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/06510-4 - Aplicação de Ressonância Magnética Nuclear (RMN) em metabolômica e metabonômica de doenças humanas
• Beneficiário: Ljubica Tasic
• Modalidade de apoio: Auxílio à Pesquisa - Regular