Exploring the compatibility between hydrothermal depolymerization of alkaline lignin from sugarcane bagasse and metabolization of the aromatics by bacteria

Autor(es)

de Menezes, Fabricia Farias ; Martim, Damaris Batistao ; Ling, Liu Yi ; Mulato, Aline Tieppo Nogueira ; Crespim, Elaine ; Oliveira, Juliana Velasco de Castro ; Driemeier, Carlos Eduardo ; de Giuseppe, Priscila Oliveira ; Rocha, George Jackson de Moraes

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 223, p. 8-pg., 2022-11-09.

Resumo

Although hydrothermal treatments for biomass fractionation have been vastly studied, their effect on the depolymerization of isolated lignins in terms of yield, composition, and compatibility of the produced lignin bio-oils with bioconversion is still poorly investigated. In this study, we evaluated the hydrothermal depolymerization of an beta-O-4'-rich lignin extracted from sugarcane bagasse by alkaline fractionation, investigating the influence of temperature (200-350 degrees C), time (30-90 min), and solid-liquid ratio (1:10-1:50 m.v(-1)) on yield of bio-oils (up to 31 wt%) rich in monomers (light bio-oils). Principal Components Analysis showed that the defunctionalization of the aromatic monomers was more pronounced in the most severe reaction conditions and that the abundance of more hydrophobic monomers increased in more diluted reactions. While the high-molecular-weight (heavy) bio-oil generated at 350 degrees C, 90 min, and 1:50 m.v(-1) failed to support bacterial growth, the corresponding light bio-oil rich in aromatic monomers promoted the growth of bacteria from 9 distinct species. The isolates Pseudomonas sp. LIM05 and Burkholderia sp. LIM09 showed the best growth performance and tolerance to lignin-derived aromatics, being the most promising for the future development of biological upgrading strategies tailored for this lignin stream. (AU)

• Processo FAPESP: 19/22213-2 - Modificação biológica de fragmentos de lignina na obtenção de compostos fenólicos
• Beneficiário: George Jackson de Moraes Rocha
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/06921-7 - Investigação das rotas metabólicas utilizadas por Xanthomonas axonopodis pv. citri para a utilização de compostos aromáticos derivados da lignina
• Beneficiário: Priscila Oliveira de Giuseppe
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 22/00474-1 - Decifrando o papel de compostos orgânicos voláteis bacterianos na promoção do crescimento e sanidade do milho (Zea mays L.) e na modulação de sua comunidade microbiana
• Beneficiário: Juliana Velasco de Castro Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/08590-8 - Identificação de vias para o catabolismo de compostos aromáticos derivados da lignina em Xanthomonas axonopodis pv citri
• Beneficiário: Damaris Batistão Martim
• Modalidade de apoio: Bolsas no Brasil - Doutorado