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Transcriptional and metabolic changes associated with internode development and reduced cinnamyl alcohol dehydrogenase activity in sorghum

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Ferreira, Savio Siqueira ; Goeminne, Geert ; Simoes, Marcella Siqueira ; de Almeida Pina, Andre Vicioli ; Alves de Lima, Leydson Gabriel ; Pezard, Jade ; Gutierrez, Ana ; Rencoret, Jorge ; Mortimer, Jenny C. ; del Rio, Jose C. ; Boerjan, Wout ; Cesarino, Igor
Número total de Autores: 12
Tipo de documento: Artigo Científico
Fonte: Journal of Experimental Botany; v. 73, n. 18, p. 27-pg., 2022-07-05.
Resumo

Phenolic metabolism is rewired during internode development and in response to cinnamyl alcohol dehydrogenase perturbation in sorghum, with different sets of genes and accumulating compounds. The molecular mechanisms associated with secondary cell wall (SCW) deposition in sorghum remain largely uncharacterized. Here, we employed untargeted metabolomics and large-scale transcriptomics to correlate changes in SCW deposition with variation in global gene expression profiles and metabolite abundance along an elongating internode of sorghum, with a major focus on lignin and phenolic metabolism. To gain deeper insight into the metabolic and transcriptional changes associated with pathway perturbations, a bmr6 mutant [with reduced cinnamyl alcohol dehydrogenase (CAD) activity] was analyzed. In the wild type, internode development was accompanied by an increase in the content of oligolignols, p-hydroxybenzaldehyde, hydroxycinnamate esters, and flavonoid glucosides, including tricin derivatives. We further identified modules of genes whose expression pattern correlated with SCW deposition and the accumulation of these target metabolites. Reduced CAD activity resulted in the accumulation of hexosylated forms of hydroxycinnamates (and their derivatives), hydroxycinnamaldehydes, and benzenoids. The expression of genes belonging to one specific module in our co-expression analysis correlated with the differential accumulation of these compounds and contributed to explaining this metabolic phenotype. Metabolomics and transcriptomics data further suggested that CAD perturbation activates distinct detoxification routes in sorghum internodes. Our systems biology approach provides a landscape of the metabolic and transcriptional changes associated with internode development and with reduced CAD activity in sorghum. (AU)

Processo FAPESP: 16/06917-1 - Análise do transcriptoma e construção de redes de co-expressão em sistemas modelo para identificação de genes envolvidos na deposição de parede celular secundária e no metabolismo de lignina em gramíneas
Beneficiário:Sávio de Siqueira Ferreira
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 15/02527-1 - Desenvolvimento de sistemas-modelo em sorgo e uso da biologia de sistemas na elucidação dos mecanismos moleculares do metabolismo fenólico e de lignina em gramíneas
Beneficiário:Igor Cesarino
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores
Processo FAPESP: 19/14604-1 - O ABC da lignificação: busca por transportadores ABC potencialmente envolvidos na deposição de lignina em Setaria viridis (L.) P.Beauv
Beneficiário:Leydson Gabriel Alves de Lima
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 15/18361-5 - Estabelecimento de um sistema de indução de diferenciação de elementos traqueais em células em suspensão de Sorghum bicolor L.
Beneficiário:Marcella Siqueira Simões
Modalidade de apoio: Bolsas no Brasil - Mestrado