| Texto completo | |
| Autor(es): |
Perin, Luiza S.
;
Moraes, Gabriela, V
;
Galeazzo, Gabriela A.
;
Oliveira, Anderson G.
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 21, p. 14-pg., 2022-11-01. |
| Resumo | |
Dinoflagellates bioluminescence mechanism depends upon a luciferin-luciferase reaction that promotes blue light emission (480 nm) in specialized luminogenic organelles called scintillons. The scintillons contain luciferin, luciferase and, in some cases, a luciferin-binding protein (LBP), which prevents luciferin from non-enzymatic oxidation in vivo. Even though dinoflagellate bioluminescence has been studied since the 1950s, there is still a lack of mechanistic understanding on whether the light emission process involves a peroxidic intermediate or not. Still, bioassays employing luminous dinoflagellates, usually from Gonyaulax or Pyrocystis genus, can be used to assess the toxicity of metals or organic compounds. In these dinoflagellates, the response to toxicity is observed as a change in luminescence, which is linked to cellular respiration. As a result, these changes can be used to calculate a percentage of light inhibition that correlates directly with toxicity. This current approach, which lies in between fast bacterial assays and more complex toxicity tests involving vertebrates and invertebrates, can provide a valuable tool for detecting certain pollutants, e.g., metals, in marine sediment and seawater. Thus, the present review focuses on how the dinoflagellates bioluminescence can be applied to evaluate the risks caused by contaminants in the marine environment. (AU) | |
| Processo FAPESP: | 19/25086-1 - Estudo do processo de bioluminescência em Harmothoe sp., Kinberg, 1856 e suas funções ecológicas |
| Beneficiário: | Gabriela Verruck de Moraes |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado Direto |
| Processo FAPESP: | 20/16142-2 - Determinação das condições de emissão de luz in vitro para novos sistemas bioluminescentes de anelídeos e sistemas que utilizam celenterazina como substrato e aplicações de sistemas bioluminescentes na detecção de poluentes de interesse marinho |
| Beneficiário: | Luíza Sandoval Perin |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |