| Texto completo | |
| Autor(es): |
Azevedo, Flavia Figueiredo
;
Cantarutti, Thiago Anselmo
;
Rosa Remiro, Paula de Freitas
;
Barbieri, Beatriz
;
Azoubel, Rafael Abboud
;
Taniguchi Nagahara, Mariana Harue
;
Moraes, Angela Maria
;
Melo Lima, Maria Helena
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | POLYMERS; v. 14, n. 21, p. 16-pg., 2022-11-01. |
| Resumo | |
The purpose of this study was to investigate tissue repair of excisional wounds in hyperglycemic animals treated with chitosan-alginate membranes (CAM) produced in the presence of glycerol. 8-week C57B1 male mice were divided into normoglycemic animals with a 0.9% saline solution topical treatment (CTSF); hyperglycemic animals with 0.9% saline solution topical treatment (DMSF) and hyperglycemic animals with glycerol-plasticized chitosan-alginate membrane topical treatment (DMCAM). On post-wound day three, the DMCAM group presented a lower number of leukocytes, mature mastocytes, a higher number of vessels (p < 0.05), and active mastocytes (p < 0.05) when compared to the CTSF and DMSF groups. There were no differences regarding the distribution, deposition, organization, and thickness of collagen fibers. On day 7 there were no differences in the analysis of fibroblasts, mastocytes, and TGF-beta 1 and VEGF expressions among the groups. Regarding collagen fibers, the DMCAM group presented slight red-orange birefringence when compared to the CTSF and DMSF groups. On day 14 there was a slight concentration of thinner elastic fibers for the DMCAM group, with a greater reorganization of papillary skin and improved red-orange birefringence collagen fibers, as well as net-shaped orientation, similar to intact skin. In addition, improved elastic fiber organization distributed in the entire neo-dermis and a larger presence of elaunin fibers were observed, in a similar pattern found in the intact skin. The use of CAM in cutaneous lesions boosted tissue repair since there was a smaller number of inflammatory cells and mastocytes, and an improvement in collagen deposition and collagen fibers. These results demonstrate the high potential of plasticized chitosan-alginate membrane for skin wound dressing of hyperglycemic patients. (AU) | |
| Processo FAPESP: | 18/05237-2 - Micronização do fármaco Imiquimode com CO2 supercrítico visando o aumento da solubilidade em meios fisiológicos e sua incorporação em novos veículos de liberação |
| Beneficiário: | Paula de Freitas Rosa Remiro |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 21/00781-9 - O gel de insulina no tratamento de lesões excisionais em camundongos hiperglicêmicos e a relação com o crescimento de folículos pilosos |
| Beneficiário: | Maria Helena de Melo Lima |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |