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Enhancement of optical properties of new purine nucleobases containing electron-donating and -withdrawing peripheral groups

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Autor(es):
Zucolotto Cocca, Leandro H. ; Pelosi, Andre G. ; Piguel, Sandrine ; Mendonca, Cleber Renato ; De Boni, Leonardo
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 234, p. 7-pg., 2022-09-01.
Resumo

Nowadays, a great deal of attention has been focused on synthesizing highly fluorescent unnatural base analogs. This has been motivated by the need to overcome the lack of fluorescence of nucleic acids' natural bases. Fluorescent unnatural base analogs, such as purines, may be used in several applications, such as DNA or RNA optical spectroscopy studies. Moreover, for purines base analogs, the optical properties, for example, emission, can be tunable through molecular engineering, improving their applications as fluorescent probes. Looking in this direction, the synthesis and optical spectroscopic studies of a new set of purines base analogs are of foremost relevance. Here, an increase in the fluorescence quantum yield was observed in molecules with NH-pi-CN arrangement. The two-photon absorption (2PA) cross-sections also increased for the lower energy 2PA state. The enhancement of both properties results in a two-photon brightness of 5 and 10 times higher than in compounds lacking the NH-pi-CN arrangement. For the higher energy 2PA state, an excited state absorption contribution to the 2PA cross-section values was observed, that was verified through ultrafast transient absorption measurements. The higher 2PA brightness makes the purines base analogs promising candidates as fluorescent probes in RNA and DNA spectroscopic studies. (AU)

Processo FAPESP: 18/11283-7 - Fotônica não linear: espectroscopia e processamento avançado de materiais
Beneficiário:Cleber Renato Mendonça
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/20886-1 - Modernização da linha de Espectroscopia Óptica Não Linear ultrarrápida: Absorção transiente e chave kerr óptica com controle da polarização.
Beneficiário:Leonardo de Boni
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/12399-0 - Aplicações de pulsos de femtossegundos em óptica não linear: espectroscopia, formatação de pulsos e microfabricação
Beneficiário:Cleber Renato Mendonça
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 15/20032-0 - Estudo das Propriedades Ópticas Não Lineares e de Transporte de Carga de Moléculas Multiramificadas via Métodos de Química Quântica.
Beneficiário:Daniel Luiz da Silva
Modalidade de apoio: Auxílio à Pesquisa - Regular