| Texto completo | |
| Autor(es): |
Lima Rassius, Alexandre Medeiro
;
Ferraz Sofia, Madruga Marcondes
;
Cardoso Victor, Gustavo Kelis
;
Teixeira, Carlos Alberto
;
Hantao, Leandro Wang
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. N/A, p. 11-pg., 2022-11-14. |
| Resumo | |
Food supplement authentication is an important concern worldwide due to the ascending consumption related to health benefits and its lack of effective regulation in underdeveloped countries, making it a target of fraudulent activities. In this context, this study evaluated fish oil supplements by comprehensive two-dimensional gas chromatography coupled to mass spectrometry (GCxGC-MS) to obtain fingerprints, which were used to build predictive models for automated authentication of the most popular products sold in Brazil. The authentication process relied on a one-class classifier model using data-driven soft independent modeling of class analogy (DD-SIMCA). The output of the model was a binary classifier: certified IFOS fish oils and non-certified ones - regardless of the source of adulteration. The compositional analysis showed a significant variation in the samples, which validated the need for reliable statistical models. The DD-SIMCA algorithm is still incipient in GCxGC studies, but it proved to be an excellent tool for authenticity purposes, achieving a chemometric model with a sensitivity of 100%, specificity of 98.6%, and accuracy of 99.0% for fish oil authentication. Finally, orthogonalized partial least square discriminant analysis (OPLS-DA) was used to identify the features that distinguished the groups, which ascertained the results of the DD-SIMCA model that IFOS-certified oils are positively correlated to omega-3 fatty acids, including eicosapentaenoic acid (EPA, C20:5 n-3) and docosahexaenoic acid (DHA, C22:6 n-3). (AU) | |
| Processo FAPESP: | 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica |
| Beneficiário: | Marco Aurelio Zezzi Arruda |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 20/01064-6 - Desenvolvimentos analíticos visando estudos com amostras reais do derrame de petróleo na costa litorânea de Pernambuco: simulações, caracterizações químicas e bioensaios de ecotoxicidade |
| Beneficiário: | Leandro Wang Hantao |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |