| Texto completo | |
| Autor(es): Mostrar menos - |
Brathwaite, Ana Clara N.
;
Alencar-Silva, Thuany
;
Carvalho, Larissa A. C.
;
Branquinho, Maryana S. F.
;
Ferreira-Nunes, Ricardo
;
Cunha-Filho, Marcilio
;
Gelfuso, Guilherme M.
;
Maria-Engler, Silvya S.
;
Carvalho, Juliana Lott
;
Silva, Joyce K. R.
;
Gratieri, Tais
Número total de Autores: 11
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Molecules; v. 27, n. 18, p. 16-pg., 2022-09-01. |
| Resumo | |
Here, we verify the depigmenting action of Pouteria macrophylla fruit extract (EXT), incorporate it into a safe topical microemulsion and assess its effectiveness in a 3D pigmented skin model. Melanocytes-B16F10- were used to assess the EXT effects on cell viability, melanin synthesis, and melanin synthesis-related gene transcription factor expression, which demonstrated a 32% and 50% reduction of intra and extracellular melanin content, respectively. The developed microemulsion was composed of Cremophor EL(R)/Span 80 4:1 (w/w), ethyl oleate, and pH 4.5 HEPES buffer and had an average droplet size of 40 nm (PdI 0.40 +/- 0.07). Skin irritation test with reconstituted epidermis (Skin Ethic RHETM) showed that the formulation is non-irritating. Tyrosinase inhibition was maintained after skin permeation in vitro, in which microemulsion showed twice the inhibition of the conventional emulsion (20.7 +/- 2.2% and 10.7 +/- 2.4%, respectively). The depigmenting effect of the microemulsion was finally confirmed in a 3D culture model of pigmented skin, in which histological analysis showed a more pronounced effect than a commercial depigmenting formulation. In conclusion, the developed microemulsion is a promising safe formulation for the administration of cutite fruit extract, which showed remarkable depigmenting potential compared to a commercial formulation. (AU) | |
| Processo FAPESP: | 18/14936-1 - Metabolismo mitocondrial ditando a heterogeneidade e a resistência do melanoma |
| Beneficiário: | Larissa Anastacio da Costa Carvalho |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/04926-6 - Melanoma e quimiorresistência: modelos in vitro e in silico para explorar alvos terapêuticos |
| Beneficiário: | Silvya Stuchi Maria-Engler |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |