In yeast, cardiolipin unsaturation level plays a key role in mitochondrial function and inner membrane integrity

Autor(es)

Luevano-Martinez, Luis Alberto ; Pinto, Isabella Fernanda Dantas ; Yoshinaga, Marcos Yukio ; Miyamoto, Sayuri

• Tipo de documento: Artigo Científico
• Fonte: BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS; v. 1863, n. 7, p. 10-pg., 2022-07-02.

Resumo

Cardiolipin is the signature phospholipid of the mitochondrial inner membrane. It participates in shaping the inner membrane as well as in modulating the activity of many membrane-bound proteins. The acyl chain composition of cardiolipin is finely tuned post-biosynthesis depending on the surrounding phospholipids to produce mature or unsaturated cardiolipin. However, experimental evidence showing that immature and mature cardiolipin are functionally equivalents for mitochondria poses doubts on the relevance of cardiolipin remod-eling. In this work, we studied the role of cardiolipin acyl chain composition in mitochondrial bioenergetics, including a detailed bioenergetic profile of yeast mitochondria. Cardiolipin acyl chains were modified by genetic and nutritional manipulation. We found that both the bioenergetic efficiency and osmotic stability of mito-chondria are dependent on the unsaturation level of cardiolipin acyl chains. It is proposed that cardiolipin remodeling and, consequently, mature cardiolipins play an important role in mitochondrial inner membrane integrity and functionality. (AU)

• Processo FAPESP: 16/12999-0 - Evolução da via de síntese da cardiolipina: implicações bioquímicas na evolução do domínio Eukarya
• Beneficiário: Luis Alberto Luevano Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/11556-2 - Cardiolipina em doenças neurodegenerativas: caracterização de produtos oxidados e de proteínas modificadas em um modelo de Esclerose Lateral Amiotrófica
• Beneficiário: Isabella Fernanda Dantas Pinto
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs