Nanocarrier-Mediated Delivery of miRNA, RNAi, and CRISPR-Cas for Plant Protection: Current Trends and Future Directions

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Mujtaba, Muhammad ; Wang, Depeng ; Carvalho, Lucas Braganca ; Oliveira, Jhones Luiz ; Santo Pereira, Anderson do Espirito ; Sharif, Rahat ; Jogaiah, Sudisha ; Paidi, Murali Krishna ; Wang, Lichen ; Ali, Qasid ; Fraceto, Leonardo Fernandes

• Tipo de documento: Artigo Científico
• Fonte: ACS AGRICULTURAL SCIENCE & TECHNOLOGY; v. 1, n. 5, p. 19-pg., 2021-10-18.

Resumo

Current trends in plant genetic transformation technologies, i.e., designing and applying molecules like miRNA, RNAi, and CRISPR-Cas, largely enable researchers to target specific sites in the plant genome to avert the growing biotic and abiotic threats to plants. However, the delivery of these molecules through conventional techniques brings an array of drawbacks such as low efficiency due to the cell wall barrier, tissue damage that leads to browning or necrosis, degradation of these biomolecules by physiological conditions (high temperature, harsh pH, and light), and plant-specific protocols. The advancements in nanotechnology offer an excellent alternative for the safe and highly efficient delivery of biomolecules such as miRNA, CRISPR-Cas, and RNAi without damaging the plant tissues. Nanoparticle (polymeric, metallic, magnetic, silica, carbon, etc.)-based delivery of biomolecules can be efficiently utilized especially for plant protection applications. Herein, we present a comprehensive overview of current trends (with a focus on the previous five years) in nanoparticle-based delivery of miRNA, RNAi, CRISPR-Cas and simillar biomolecules for plant protection applications. In addition, a future perspective focuses on the research gaps and unexplored potentials of nanoparticles for the delivery of biomolecules. (AU)

• Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
• Beneficiário: Leonardo Fernandes Fraceto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/21142-1 - Encapsulamento de Bacillus thuringiensis (Bacillales: Bacillaceae) em micropartículas poliméricas e associação com agente químico nanoencapsulado: avaliação da atividade biológica e mecanismos de ação
• Beneficiário: Jhones Luiz de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/23608-8 - Desenvolvimento de nanopesticidas marcados com sondas fluorescentes para estudos de mecanismo de ação e toxicidade
• Beneficiário: Lucas Bragança de Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado