| Texto completo | |
| Autor(es): |
Ferigolo, Leticia F.
;
Vicente, Mateus H.
;
Nogueira, Fabio T. S.
Número total de Autores: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLASMID; v. 121, p. 8-pg., 2022-04-12. |
| Resumo | |
Gateway system is one of the most known cloning systems, which makes it compatible with several expression vectors, including those used for Yeast Two-Hybrid (Y2H) and Bimolecular Fluorescence Complementation (BiFC) assays. However, this system is laborious and expensive due to its two-step cloning. In this research, we developed a new cloning strategy named Brick into the Gateway (BiG). This approach uses GoldenBraid/Gate assemblies to create a DNA fragment of interest flanked by attL sites, which can be directly recombined into Gateway destination vectors. BiG method showed a high recombination efficiency and ensured the correct reading frame, which was successfully tested in Y2H and BiFC assays. BiG has proven to be a rapid, low-cost, reusable, and directional cloning method which allows the merged use of systems. (AU) | |
| Processo FAPESP: | 19/20157-8 - Avaliação de possíveis interações entre as vias de florescimento fotoperiódica, age e giberelina com o módulo SP/SFT e seus impactos sobre a arquitetura do tomateiro (Solanum lycopersicum L.) |
| Beneficiário: | Mateus Henrique Vicente |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 18/17441-3 - Controle molecular da arquitetura vegetal: interconexão entre microRNAs, fatores de transcrição e fitohormônios |
| Beneficiário: | Fabio Tebaldi Silveira Nogueira |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |