Humanized mice for investigating sustained Plasmodium vivax blood-stage infections and transmission

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Luiza-Batista, Camilla ; Thiberge, Sabine ; Serra-Hassoun, Malika ; Nardella, Flore ; Claes, Aurelie ; Nicolete, Vanessa C. ; Commere, Pierre-Henri ; Mancio-Silva, Liliana ; Ferreira, Marcelo U. ; Scherf, Artur ; Garcia, Sylvie

• Tipo de documento: Artigo Científico
• Fonte: NATURE COMMUNICATIONS; v. 13, n. 1, p. 9-pg., 2022-07-15.

Resumo

Plasmodium vivax is the most widespread human malaria parasite. Due to the presence of extravascular reservoirs and relapsing infections from dormant liver stages, P. vivax is particularly difficult to control and eliminate. Experimental research is hampered by the inability to maintain P. vivax cultures in vitro, due to its tropism for immature red blood cells (RBCs). Here, we describe a new humanized mice model that can support efficient human erythropoiesis and maintain long-lasting multiplication of inoculated cryopreserved P. vivax parasites and their sexual differentiation, including in bone marrow. Mature gametocytes were transmitted to Anopheles mosquitoes, which led to the formation of salivary gland sporozoites. Importantly, blood-stage P. vivax parasites were maintained after the secondary transfer of fresh or frozen infected bone marrow cells to naive chimeras. This model provides a unique tool for investigating, in vivo, the biology of intraerythrocytic P. vivax. Plasmodium vivax is the most widespread human malaria parasite but due to its tropism for immature red blood cells in vitro culturing and studies are difficult. Here, LuizaBatista et al. generate a humanized mouse supporting human erythropoiesis and allowing asexual and sexual development of P. vivax in bone marrow and peripheral blood, as well as transmission to mosquitoes. (AU)

• Processo FAPESP: 16/18740-9 - Bases científicas para a eliminação da malária residual na Amazônia Brasileira
• Beneficiário: Marcelo Urbano Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Temático