Polycaprolactone scaffolds as a biomaterial for cementoblast delivery: An in vitro study

Autor(es)

Abdalla, Henrique B. ; Marchioro, Rayssa R. ; Galvao, Karen Elizabeth A. ; Teixeira, Lucas N. ; Kantovitz, Kamila R. ; Millas, Ana Luiza G. M. ; Nociti Jr, Francisco H.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PERIODONTAL RESEARCH; v. 57, n. 5, p. 10-pg., 2022-08-05.

Resumo

Objective To define the potential of polycaprolactone (PCL) scaffold for cementoblast delivery. Background Dental cementum is critical for tooth attachment and position, and its regenerative capabilities remain unpredictable. Methods PCL scaffolds were manufactured by the electrospinning technique at 10% and 20% (w/v) and seeded with cementoblasts (OCCM-30). Scaffolds were characterized for their morphology and biological performance by scanning electron microscopy (SEM), confocal and conventional histology, cytocompatibility (PrestoBlue assay), gene expression (type I collagen - Col1; bone sialoprotein - Bsp; runt-related transcription factor 2 - Runx-2; alkaline phosphatase - Alpl; osteopontin - Opn; osteocalcin - Ocn, osterix - Osx), and the potential to induce extracellular matrix deposition and mineralization in vitro. Results Overall, data analysis showed that PCL scaffolds allowed cell adhesion and proliferation, modulated the expression of key markers of cementoblasts, and led to enhanced extracellular matrix deposition and calcium deposition as compared to the control group. Conclusion Altogether, our findings allow concluding that PCL scaffolds are a viable tool to culture OCCM-30 cells, leading to an increased potential to promote mineralization in vitro. Further studies should be designed in order to define the clinical relevance of cementoblast-loaded PCL scaffolds to promote new cementum formation. (AU)

• Processo FAPESP: 19/04276-7 - O uso de microagulhas revestíveis com fármacos para o controle de da dor e inflamação
• Beneficiário: Henrique Ballassini Abdalla
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/05274-8 - Bioimpressão 3D de enxertos vasculares e as biotintas utilizadas na aplicação
• Beneficiário: Ana Luíza Garcia Millás Massaguer
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisa Inovativa em Pequenas Empresas - PIPE