| Texto completo | |
| Autor(es): |
Leroy, Kaat
;
Vilas-Boasa, Vania
;
Gijbels, Eva
;
Vanderborght, Bart
;
Devisscher, Lindsey
;
Cogliati, Bruno
;
Van den Bossche, Bert
;
Colle, Isabelle
;
Vinken, Mathieu
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | EXCLI JOURNAL; v. 21, p. 19-pg., 2022-01-01. |
| Resumo | |
Connexin proteins can form hexameric hemichannels and gap junctions that mediate paracrine and direct intercel-lular communication, respectively. Gap junction activity is crucial for the maintenance of hepatic homeostasis, while connexin hemichannels become particularly active in liver disease, such as hepatitis, fibrosis, cholestasis or even hepatocellular carcinoma. Channels consisting of connexin-like proteins named pannexins have been directly linked to liver inflammation and cell death. The goal of the present study was to characterize the expression and subcellular localization of connexins and pannexins in liver of patients suffering from various chronic and neo-plastic liver diseases. Specifically, real-time quantitative reverse transcription polymerase chain reaction, im-munoblotting and immunohistochemistry analyses were performed on human liver biopsies. It was found that pannexin1 and pannexin2 gene expression are correlated to a certain degree, as is pannexin1 protein expression with connexin32 and connexin43 protein expression. Furthermore, this study is the first to detect pannexin3 in human patient liver biopsies via both immunoblot and immunohistochemistry. (AU) | |
| Processo FAPESP: | 18/10953-9 - As conexinas, panexinas e seus hemi(canais) são novos biomarcadores e alvos terapêuticos no prognóstico e terapia do câncer hepático? |
| Beneficiário: | Bruno Cogliati |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |