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Dilution strategy to obtain H-2 in the 2nd acidogenic phase from enzymatically pre-treated sugarcane bagasse

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Autor(es):
Silva Rabelo, Camila A. B. ; Dionizio, Bruna S. ; Moura, Alana G. L. ; Malago-, Wilson, Jr. ; Varesche, Maria Bernadete A.
Número total de Autores: 5
Tipo de documento: Artigo Científico
Fonte: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 47, n. 76, p. 18-pg., 2022-09-05.
Resumo

Sugarcane bagasse (SCB) pre-treated with laccase was used as substrate in two acidogenic phases (1 P and 2 P). In 1 P, the pretreatment conditions were statistically optimized (pH 4.0, 37 degrees C, 0.4 U of laccase/mL, 24.0 g SCB/L and 120 min of reaction), which allowed obtaining 84% more H-2 (166.8 mL/L) than SCB in natura (90.4 mL/L). In 2 P, the liquid fraction of the 1 P reactors was diluted (90%) and more 108.5 ml H-2/L was obtained. Furthermore, the quantification of enzymes genes responsible for the hydrolysis (Cel) and H-2 production (Hyd) was carried out. An increase in the expression of both genes was observed from the phase of highest H-2 production rates in P1. In 2 P, the 1 P low genic expression was indicative of the autochthonous bacteria activity. The 2nd acidogenic phase strategy proved to be a promising novelty for the use of pre-hydrolyzed substrates with concomitant production of more value-added products. (c) 2022 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 15/06246-7 - Aplicação do conceito de biorrefinaria a estações de tratamento biológico de águas residuárias: o controle da poluição ambiental aliado à recuperação de matéria e energia
Beneficiário:Marcelo Zaiat
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 18/26470-7 - Influência da sacarificação do bagaço de cana-de-açúcar por lacase na produção fermentativa de biocombustíveis
Beneficiário:Camila Abreu Borges da Silva Rabelo
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado