| Texto completo | |
| Autor(es): |
Guimaraes, Camila Carla
;
Sandy, Camila Mira
;
Figueira, Rebeca
;
Goncalves, Mirani da Rocha
;
Crevelin, Eduardo Jose
;
Cardoso, Pedro Henrique
;
Fabro, Alexandre Todorovic
;
Carmona, Fabio
;
Borges, Marcos de Carvalho
;
Soares Pereira, Ana Maria
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Ethnopharmacology; v. 302, p. 11-pg., 2023-02-10. |
| Resumo | |
Ethnopharmacological relevance: Stachytarpheta cayennensis (Verbenaceae) has been used in Brazilian traditional medicine to treat asthma and other respiratory diseases. Aims of the study: To investigate the effects of different doses of standardized hydro-ethanolic (SCH) and aqueous (SCA) extracts of aerial parts of S. cayennensis using a murine ovalbumin (OVA)-induced asthma model.Materials and methods: The major constituents of the plant extracts were identified and standardized by ultra -performance liquid chromatography coupled with mass spectrometry. Balb/c mice were challenged with OVA solution and treated concomitantly by intraperitoneal injection of standardized SCH or SCA extracts at 50, 100, and 200 mg/kg concentrations. OVA-challenged control animals were treated with either dexamethasone (OVA-DEX) or saline solution (OVA-SAL). After challenge, we assessed in vivo bronchial hyperresponsiveness, airway inflammation (number of cells), peribronchial inflammation (histological analysis) and production of OVA -specific IgE and interleukin (IL)-4, IL-5, and IL-13 (ELISA).Results: Acteoside, isoacteoside, and ipolamiide were the major constituents of SCH and SCA. The respective concentrations of acteoside in SCH and SCA were 78 and 98 mu g/mL, while those of ipolamiide were 30 and 19 mu g/mL. Treatment with 200 mg/kg of SCH or SCA decreased IL-4, IL-5, and IL-13 in lung homogenates. These reductions were accompanied by a lower influx of inflammatory cells (eosinophils, lymphocytes, and macro-phages) to the airways and lungs. In addition to the anti-inflammatory effects, administration of SCA, but not SCH, ameliorated the parameters of bronchial hyperresponsiveness and decreased levels of circulating OVA -specific IgE.Conclusion: The results presented herein demonstrate for the first time the anti-asthmatic activity of S. cayennensis extracts in a murine model, thereby supporting the ethnopharmacological uses of the plant. (AU) | |
| Processo FAPESP: | 10/20600-4 - Estudo da prevenção e tratamento da asma induzida por alérgeno em um modelo animal com a administração de Saccharomyces cerevisiae vivas e inativadas pelo calor |
| Beneficiário: | Marcos de Carvalho Borges |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores |