| Texto completo | |
| Autor(es): |
da Costa, Jean Lucas Fernandes
;
Furino, Vanessa de Oliveira
;
de Castro, Cynthia Aparecida
;
Alves, Joao Manoel
;
Duarte, Ana Claudia Garcia de Oliveira
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN PHYSIOLOGY; v. 13, p. 10-pg., 2022-03-24. |
| Resumo | |
This study aimed to determine the concentrations of inflammatory markers in visceral adipose tissue (VAT) and skeletal muscle, and changes in body mass and adipocyte size in diet-induced obese rats after moderate-intensity continuous training (MICT) and/or dietary intervention. After 8 weeks of obesity induction through a high-fat diet (HFD) consumption, twenty diet-induced obese male Wistar rats were divided into four groups as follows: (i) control rats fed with HFD (HFD-SED), (ii) obese rats fed with HFD and submitted to MICT (HFD-MICT), (iii) obese rats that were submitted to a nutritional intervention by switching HFD to chow diet (CD-SED), and (iv) obese rats that were submitted to MICT and nutritional intervention (CD-MICT). All the animals in the training groups were submitted to MICT, with an intensity of 50-85% of V-max, 60 min/day, 3 days/week for 8 weeks. Gastrocnemius muscle (GAST) and mesenteric adipose tissue (mWAT) were collected to quantify tumor necrosis factor alpha (TNF-alpha), interleukin (IL)-6, and IL-10 using ELISA. The body mass was recorded before and after the experimental protocols, and the adipocyte morphology was assessed using histological analysis. The results showed that HFD-SED had higher body mass, higher concentrations of inflammatory markers in mWAT, and higher increase in adipocyte size. The CD-SED and CD-MICT groups presented with reduced body mass, relative weight of mWAT, and adipocyte size. Moreover, the inflammatory markers in mWAT were reduced after dietary intervention (TNF-alpha), MICT (IL-10 and TNF-alpha), or both interventions combined (IL-6 and TNF-alpha). In contrast, there was no reduction in GAST-relative weight or concentrations of inflammatory markers for any treatment. Finally, we concluded that 8 weeks of dietary intervention alone and combined with MICT were effective in reducing some of the deleterious effects caused by obesity. (AU) | |
| Processo FAPESP: | 17/09602-4 - Estudo longitudinal dos marcadores metabólicos e fisiológicos no desenvolvimento da obesidade e síndrome metabólica em ratos alimentados com dieta hiperlipídica |
| Beneficiário: | Ana Cláudia Garcia de Oliveira Duarte |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |