A Microwave-Based Microfluidic Cell Detecting Biosensor for Biological Quantification Using the Metallic Nanowire-Filled Membrane Technology

Autor(es)

Shahri, Atena Amanati ; Omidvar, Amir Hossein ; Rehder, Gustavo Pamplona ; Caniato Serrano, Ariana Lacorte

• Tipo de documento: Artigo Científico
• Fonte: SENSORS; v. 22, n. 9, p. 13-pg., 2022-05-01.

Resumo

A label-free, sensitive, miniaturized sensing device was developed for detecting living cells in their flow stream. The outstanding performance of this biosensor in distinguishing living cells in cell suspension was achieved by integrating microstrip stub resonator above a microfluidic structure using the metallic nanowire-filled membrane technology. The cell suspension flows in a microfluidic channel placed between the tip of the stub resonator and its ground plane as the substrate to take advantage of the uniform and concentrated field distribution. We studied the changes in relative permittivity due to the presence of a single living cell in the phase of the transmitted signal (S-21). An average variation of as much as 22.85 +/- 1.65 degrees at similar to 11.1 GHz is observed for the living cell sensing using this optimized device. This biosensor could detect rapid flowing cells in their biological medium in real-time and hence, can be used as an early diagnosis and monitoring tool for diseases. (AU)

• Processo FAPESP: 16/25779-9 - EMU concedido no processo 2012/15159-2: Analisador Vetorial de Rede (VNA) até 70 GHz
• Beneficiário: Ariana Maria da Conceicao Lacorte Caniato Serrano
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais
• Beneficiário: Mauro Bertotti
• Modalidade de apoio: Auxílio à Pesquisa - Temático