Photobiomodulation and B vitamins administration produces antinociception in an orofacial pain model through the modulation of glial cells and cytokines expression

Autor(es)

Martins, D. O. ; Marques, D. P. ; Venega, R. A. G. ; Chacur, M.

• Tipo de documento: Artigo Científico
• Fonte: BRAIN, BEHAVIOR, & IMMUNITY - HEALTH; v. 2, p. 9-pg., 2020-03-01.

Resumo

Chronic constriction injury (CCI) of infraorbital nerve (IoN) results in whisker pad mechanical allodynia in rats and activation glial cells contributing to the development of orofacial pain. Whisker pad mechanical allodynia (von Frey stimuli) was tested pre and postoperatively and conducted during the treatment time. Photo-biomodulation (PBM) and vitamins B complex (VBC) has been demonstrated therapeutic efficacy in ameliorate neuropathic pain. The aim of this study was to evaluate the antinociceptive effect of PBM, VBC or the combined treatment VBC thorn PBM on orofacial pain due to CCI-IoN. Behavioral and molecular approaches were used to analyses nociception, cellular and neurochemical alterations. CCI-IoN caused mechanical allodynia and cellular alterations including increased expression of glial fibrillary acid protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba-1), administration of VBC (B1/B6/B12 at 180/180/1.8 mg/kg, s.c., 5 times all long 10 sessions) and PBM therapy (904 nm, power of 75Wpico, average power of 0.0434 W, pulse frequency of 9500 Hz, area of the beam 0.13 cm2, 18 s duration, energy density 6 J/cm2, with an energy per point of 0.78 J for 10 sessions) or their combination presented improvement of the nociceptive behavior and decreased expression of GFAP and Iba-1. Additionally, CCI-IoN rats exhibited an upregulation of IL1 & beta;, IL6 and TNF-& alpha; expression and all treatments prevented this upregulation and also increased IL10 expression. Overall, the present results highlight the pain reliever effect of VBC or PBM alone or in combination, through the modulation of glial cells and cyto-kines expression in the spinal trigeminal nucleus of rats. (AU)

• Processo FAPESP: 14/24533-0 - Terapia fotodinâmica na neuralgia do trigêmeo
• Beneficiário: Marucia Chacur
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/24256-0 - Estudo das estruturas envolvidas na dor orofacial e perspectivas do tratamento combinado de laser de baixa intensidade e vitaminas do complexo B em modelo de neuralgia trigeminal
• Beneficiário: Daniel de Oliveira Martins
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado