| Texto completo | |
| Autor(es): |
Spriano, S.
;
Riccucci, G.
;
Orlygsson, G.
;
Ng, C. H.
;
Verne, E.
;
Sehn, F. P.
;
de Oliveira, P. T.
;
Ferraris, S.
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | SURFACE & COATINGS TECHNOLOGY; v. 470, p. 11-pg., 2023-10-15. |
| Resumo | |
Two bioactive glasses were coated with chitosan: SCNB belongs to the SiO2-CaO-Na2O system, and SCNA has the addition of Al2O3 to enhance chemical stability. Different coating methods were compared: direct physical attachment, surface activation through tresyl chloride, and polydopamine as a linker. The samples were char-acterized through SEM-EDS, contact angle measurements, FTIR, zeta potential titrations, tape tests, in vitro precipitation of hydroxylapatite (bioactivity), and cell cultures (RAW 264.7 and UMR-106) on some selected samples. Direct physical attachment (in acetic acid) or use of polydopamine allowed complete surface coverage, while it dropped to one-quarter on both glasses by using tresyl chloride. The coating had a contact angle of about 80 degrees and it well showed typical functional groups (FTIR). The coatings on SCNA were chemically and mechan-ically stable (classified as 4-5B by the tape tests, partially maintained after soaking for 14 days), and showed an isoelectric point around 8. On SCNB, the coatings were unstable (classified as 0-3B, dissolved during soaking) but bioactivity was preserved. The coating affected the biological outcome of SCNA with M0/M1 polarization (1 day) and reduced viability of macrophages (3 days), while osteoblastic cells showed poor adhesion but maintained cell viability and osteogenic potential (3-7 days). (AU) | |
| Processo FAPESP: | 16/50298-4 - Funcionalização de superfície de materiais bioativos com moléculas naturais para a modulação da resposta tecidual a implantes aloplásticos |
| Beneficiário: | Paulo Tambasco de Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |