Lactobacillus acidophilus impairs the establishment of pathogens in a subgingival multispecies biofilm

Autor(es)

Bueno, Manuela Rocha ; Dudu-Silva, Gustavo ; Macedo, Tatiane Tiemi ; Gomes, Aline Paim de Abreu Paulo ; Braga, Arthur Rodrigues Oliveira ; Aguiar Silva, Lucas Daylor ; Bueno-Silva, Bruno

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN DENTAL MEDICINE; v. 4, p. 7-pg., 2023-07-31.

Resumo

The present study evaluated the antibiofilm effects of Lactobacillus acidophilus within a subgingival multispecies biofilm. Lactobacillus acidophilus (La5) at 1 x 10(2), 1 x 10(4), and 1 x 10(6) were included at the beginning of biofilm formation, which lasted 7 days. The biofilms comprised 33 periodontitis-related bacterial species and the Calgary Biofilm device was used. At the end, DNA-DNA hybridization (checkerboard) was performed. A Kruskal-Wallis test followed by a Dunn post hoc test were performed (p & LE; 0.05). La5 at 1 x 10(4) and 1 x 10(6) reduced the total counts of biofilm and the proportions of red and green complexes when compared to the control biofilm without La5 (p & LE; 0.05). La5 at 1 x 10(4) increased the proportions of Actinomyces complex compared to the controls (p & LE; 0.05). Both La5 at 1 x 10(4) and 1 x 10(6) decreased levels of 20 and 14 distinct species, respectively, including Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum polymorphum, and Parvimonas micra compared to the control (p & LE; 0.05). Only La5 at 1 x 10(4) reduced the levels of Tannerella forsythia, Fusobacterium periodonticum, and Aggregatibacter actinomycetencomytans compared to the control (p & LE; 0.05). L. acidophilus inhibited establishing periodontic pathogens from red complex such as P. gingivalis and T. forsythia in a subgingival multispecies biofilm. (AU)

• Processo FAPESP: 17/16377-7 - Avaliação do efeito de bactérias probióticas sobre a resposta imune contra a.actinomycetemcomitans: estudo em células epiteliais gengivais e em modelo experimental de infecção in vivo.
• Beneficiário: Manuela Rocha Bueno
• Modalidade de apoio: Bolsas no Brasil - Doutorado