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Dona Rodrigues Almeida, Gustavo Henrique
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da Silva-Junior, Leandro Norberto
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Gibin, Mariana Sversut
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dos Santos, Henrique
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de Oliveira Horvath-Pereira, Bianca
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Mazo Pinho, Leticia Beatriz
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Baesso, Mauro Luciano
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Sato, Francielle
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Hernandes, Luzmarina
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Long, Charles R.
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Relly, Luciana
;
Miglino, Maria Angelica
;
Oliveira Carreira, Ana Claudia
Número total de Autores: 13
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| Tipo de documento: | Artigo Científico |
| Fonte: | CELLS; v. 12, n. 14, p. 20-pg., 2023-07-01. |
| Resumo | |
The application of decellularized scaffolds for artificial tissue reconstruction has been an approach with great therapeutic potential in regenerative medicine. Recently, biomimetic ovarian tissue reconstruction was proposed to reestablish ovarian endocrine functions. Despite many decellularization methods proposed, there is no established protocol for whole ovaries by detergent perfusion that is able to preserve tissue macro and microstructure with higher efficiency. This generated biomaterial may have the potential to be applied for other purposes beyond reproduction and be translated to other areas in the tissue engineering field. Therefore, this study aimed to establish and standardize a protocol for porcine ovaries' decellularization based on detergent perfusion and ultrasonication to obtain functional whole-ovary scaffolds. For that, porcine ovaries (n = 5) were perfused with detergents (0.5% SDS and 1% Triton X-100) and submitted to an ultrasonication bath to produce acellular scaffolds. The decellularization efficiency was evaluated by DAPI staining and total genomic DNA quantification. ECM morphological evaluation was performed by histological, immunohistochemistry, and ultrastructural analyses. ECM physico-chemical composition was evaluated using FTIR and Raman spectroscopy. A cytocompatibility and cell adhesion assay using murine fibroblasts was performed. Results showed that the proposed method was able to remove cellular components efficiently. There was no significant ECM component loss in relation to native tissue, and the scaffolds were cytocompatible and allowed cell attachment. In conclusion, the proposed decellularization protocol produced whole-ovaries scaffolds with preserved ECM composition and great potential for application in tissue engineering. (AU) | |
| Processo FAPESP: | 14/50844-3 - Matriz extracelular na saúde e matriz placentária na regeneração de tecidos |
| Beneficiário: | Maria Angelica Miglino |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 21/05445-7 - Fábrica de tecidos funcionalizados: bioengenharia baseada nas interações da matriz extracelular com biopolímeros e bioimpressão |
| Beneficiário: | Maria Angelica Miglino |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |