Pterodon emarginatus: Antinociceptive Synergistic Effect of Geranylgeraniol and Vouacapans Fractions

Autor(es)

Basting, Rosanna Tarkany ; Grando, Rogerio ; Spindola, Humberto Moreira ; Tinti, Sirlene Valerio ; Queiroz, Nubia de Cassia Almeida ; Sousa, Ilza Maria de Oliveira ; de Carvalho, Joao Ernesto ; Saad, Sara Teresinha Olalla ; Foglio, Mary Ann

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 33, n. 4, p. 7-pg., 2023-06-07.

Resumo

Synergy considers that a plant extract or combination of compounds together is more beneficial than an isolated compound. Pterodon emarginatus Vogel, Fabaceae, fruits are popularly used due to their anti-inflammatory activities, which have relationship with diterpenes furans (vouacapans) and geranylgeraniol. This study evaluated the pharmacological interactions of a sesquiterpenes (a-humulene and trans-caryophyllene, fraction A) enriched fraction, a geranylgeraniol (fraction B), and a vouacapans enriched fraction (fraction C) obtained from the dichloromethane extract, tested on an antinociceptive model. First, all individual fractions had their median effective dose (ED50) determined in abdominal writhing test induced by acetic acid. Fractions A and B gave ED50 values of 102.34 and 101.96 mg/kg, respectively, whereas fraction C provided the lowest ED50 value of 63.83 mg/kg. When fractions A and C (3:1) were associated, both at their ED50 concentration, the maximum effect previously estimated was not achieved on the isobologram, indicating an antagonism action. Nevertheless, the association of those both fractions in 1:1 or 1:3 ratio demonstrated an increase in antinociceptive activity, classified as synergistic effect. The combination of fractions B and C (3:1; 1:1; and 1:3) significantly reduced abdominal writhing's, classified as synergistic interactions. The combination of acyclic diterpene fraction (Fraction B) with vouacapans fraction (Fraction C) demonstrated a synergistic pharmacological outcome. (AU)

• Processo FAPESP: 15/08600-2 - Pterodon pubescens Benth. e Cordia verbenacea DC: avaliação do efeito sinérgico na atividade anti-inflamatória e estudos de mecanismos de ação e sinalização celular
• Beneficiário: Rosanna Tarkany Basting
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 18/20252-8 - Avaliação da associação de extratos de frutos de Pterodon pubescens Benth., Arrabidaea chica Verlot e Bixa orellana nas atividades cicatrizantes, antinociceptiva e anti-inflamatória
• Beneficiário: Mary Ann Foglio
• Modalidade de apoio: Auxílio à Pesquisa - Regular