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Comparative Genomics of Pesticide-Degrading Enterococcus Symbionts of Spodoptera frugiperda (Lepidoptera: Noctuidae) Leads to the Identification of Two New Species and the Reappraisal of Insect-Associated Enterococcus Species

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Autor(es):
Gomes, Ana Flavia Freitas ; de Almeida, Luis Gustavo ; Consoli, Fernando Luis
Número total de Autores: 3
Tipo de documento: Artigo Científico
Fonte: MICROBIAL ECOLOGY; v. N/A, p. 23-pg., 2023-07-11.
Resumo

Enterococcus species have been described as core members of the microbial community of Spodoptera frugiperda (Lepidoptera: Noctuidae) and have been previously reported as insecticide degrading agents. This study aimed to investigate the molecular composition of these microbial symbionts of S. frugiperda to better understand their association with the host and their potential for insecticide metabolization. Through phenotypic assays and comparative genomic analyses of several pesticide-degrading Enterococcus isolated from the gut of S. frugiperda larvae, we identified two new species: Enterococcus entomosocium n. sp. and Enterococcus spodopteracolus n. sp. Their identities as new species were confirmed by whole genome alignment, utilizing cut-offs of 95-96% for the average nucleotide identity (ANI) and 70% for the digital DNA: DNA hybridization (dDDH) values. The systematic positioning of these new species within the genus Enterococcus was resolved using genome-based analysis, revealing Enterococcus casseliflavus as a sister group of E. entomosocium n. sp., and Enterococcus mundtii as a sister group of E. spodopteracolus n. sp. Comparative genomic analyses of several isolates of E. entomosocium n. sp. and E. spodopteracolus n. sp. provided a better assessment of the interactions established in the symbiotic association with S. frugiperda and led to the discovery of misidentified new species of Enterococcus associated with insects. Our analyses indicated that the potential of E. entomosocium n. sp. and E. spodopteracolus n. sp. to metabolize different pesticides arises from molecular mechanisms that result in rapid evolution of new phenotypes in response to environmental stressors, in this case, the pesticides their host insect is exposed to. (AU)

Processo FAPESP: 10/13714-3 - Simbiontes de Insetos e a Degradação de Xenobióticos: Implicações no Desenvolvimento de Resistência de Insetos a Inseticidas e seu Potencial Biotecnológico para Biorremediação
Beneficiário:Luis Gustavo de Almeida
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 18/21155-6 - Compreensão da evolução da resistência a inseticidas em pragas agrícolas do Brasil: rumo a estratégias efetivas de manejo da resistência a inseticidas (MRI)
Beneficiário:Celso Omoto
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 11/50877-0 - Diversidade, ecologia e potencial biotecnológico da bacteriofauna simbionte associada a insetos
Beneficiário:Fernando Luis Cônsoli
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático