Enhanced mucosal response in sheep harbouring beta(A) beta-globin against haemonchosis

Autor(es)

Okino, Cintia Hiromi ; Niciura, Simone Cristina Meo ; Giaretta, Paula Roberta ; Melito, Glaucia Roberta ; Kapritchkoff, Rafaela Tami Ikeda ; dos Santos, Isabella Barbosa ; Rech, Raquel Rubia ; Minho, Alessandro Pelegrine ; Esteves, Sergio Novita ; Chagas, Ana Carolina de Souza

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. N/A, p. 12-pg., 2023-07-03.

Resumo

Association between ovine beta-globin polymorphisms and resistance against haemonchosis was described and related to the mechanism of high oxygen affinity beta(A) -> beta(C) switch during anaemia, but there are no studies regarding the involved local host responses. Phenotypic parameters and local responses were evaluated in sheep from two beta-globin haplotypes naturally infected with Haemonchus contortus. Morada Nova lambs were monitored at 63, 84 and 105 days of age for faecal egg counts and packed cell volume (PCV) under natural infection with H. contortus. At 210 days of age, lambs of Hb-AA and Hb-BB beta-globin haplotypes were euthanised, and the fundic region of abomasum was sampled for evaluation of microscopic lesions and relative expression of genes related to immune, mucin and lectin activities. Lambs harbouring the beta(A) allele presented an improved resistance/resilience against clinical haemonchosis, showing higher PCV during infection. Hb-AA animals presented increased eosinophilia in the abomasum compared to Hb-BB animals, accompanied by higher Th2 profile, mucin and lectin activity transcripts, while the inflammatory response was increased in Hb-BB animals. This is the first report to demonstrate an enhanced local response in the primary site of H. contortus infection related to beta(A) allele of beta-globin haplotype. (AU)

• Processo FAPESP: 22/00776-8 - Determinação da relação entre haplótipos da ²-globina ovina e resistência à infecção por Haemonchus contortus nas raças Santa Inês, Texel e White Dorper
• Beneficiário: Rafaela Tami Ikeda Kapritchkoff
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 21/02535-5 - Abordagem parasita-hospedeiro-ambiente para controle da resistência anti-helmíntica em rebanhos ovinos
• Beneficiário: Ana Carolina de Souza Chagas
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/16070-1 - Estudo do impacto da infecção por nematóides gastrintestinais no desempenho de Cordeiros Morada Nova para desenvolvimento de método seletivo de controle parasitário
• Beneficiário: Isabella Barbosa dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/02929-3 - Estratégia de tratamento anti-helmíntico seletivo baseado no ganho de peso em cordeiros Morada Nova
• Beneficiário: Ana Carolina de Souza Chagas
• Modalidade de apoio: Auxílio à Pesquisa - Regular