Breastfeeding lifespan control of growth, maintenance, and metabolism of small intestinal epithelium

Autor(es)

da Costa, Aline Vasques ; Rattes, Isadora Campos ; Goes, Carolina Purcell ; Lobo, Larissa Honda Greco ; Barreto, Laylla Barreto e ; Gama, Patricia

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Physiology; v. N/A, p. 12-pg., 2023-08-09.

Resumo

Gastrointestinal epithelial cells respond to milk-born molecules throughout breastfeeding, influencing growth, and development. The rapid renewal of the small intestine depends on the proliferation in the crypt that drives cell fates. We used early weaning model to investigate immediate and late effects of breastfeeding on proliferation, differentiation of jejunal epithelial cells. Wistar rats were either allowed to suckle (S) until 21 postnatal days or submitted to early weaning (EW) at 15 days. By comparing ages (18, 60, and 120 days), we found that EW decreased Ki67 indices and villi height at 18 and 60 days (p < 0.05), and at 120 days they were similar between diets. Proliferative reduction and augmented expression of Cdkn1b (p27 gene) were parallel. In the stem cell niche, EW increased the number and activity (Defa24) of Paneth cells at 18 and 60 days (p < 0.05), and Lgr5 and Ascl2 genes showed inverted responses between ages. Among target cells, EW decreased goblet cell number at 18 and 60 days (p < 0.05) and increased it at 120 days (p < 0.05), whereas enteroendocrine marker genes were differentially altered. EW reduced enterocytes density at 18 days (p < 0.05), and at 120 days this population was decreased (vs. 60 days). Among cell fate crypt-controlling genes, Notch and Atoh1 were the main targets of EW. Metabolically, intraperitoneal glucose tolerance was immediately reduced (18 days), being reverted until 120 days (p < 0.05). Currently, we showed that breastfeeding has a lifespan influence on intestinal mucosa and on its stem cell compartment. We suggest that, although jejunum absorptive function is granted after early weaning, the long lasting changes in gene expression might prime the mucosa with a different sensitivity to gut disorders that still have to be further explored. (AU)

• Processo FAPESP: 18/07409-5 - Efeito do desmame precoce sobre marcadores moleculares e desenvolvimento da mucosa intestinal
• Beneficiário: Aline Vasques da Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/07782-8 - Efeito do desmame precoce sobre marcadores moleculares durante o crescimento e desenvolvimento pós- natal da mucosa intestinal do rato.
• Beneficiário: Patrícia Gama
• Modalidade de apoio: Auxílio à Pesquisa - Regular