| Texto completo | |
| Autor(es): |
Grosso Neves, Lia Mara
;
Parizotto, Nivaldo Antonio
;
Tim, Carla Roberta
;
Floriano, Elaine Medeiros
;
Vianna Lopez, Renata F.
;
Venancio, Tiago
;
Fernandes, Joao Batista
;
Cominetti, Marcia Regina
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | WOUND REPAIR AND REGENERATION; v. 28, n. 5, p. 11-pg., 2020-06-26. |
| Resumo | |
Prolonged skin exposure to ultraviolet radiation (UVR) induces premature aging in both the epidermis and the dermis. Chronic exposure to UVR induces the activation of mitogen-activated protein kinase (MAPK) signaling pathway, activating c-Jun, c-Fos expression, and transcription factor of AP-1 activating protein. AP-1 activation results in the positive induction of matrix metalloproteinase (MMP) synthesis, which degrade skin collagen fibers. Polysaccharides from the fruit ofLycium barbarum(LBP fraction) have a range of activities and have been demonstrate to repair the photodamage. In different approaches, laser application aims to recover the aged skin without destroying the epidermis, promoting a modulation, called photobiomodulation (PBM), which leads to protein synthesis and cell proliferation, favoring tissue repair. Here we developed a topical hydrogel formulation from a polysaccharide-rich fraction ofLycium barbarumfruits (LBP). This formulation was associated with PBM (red laser) to evaluate whether the isolated and combined treatments would reduce the UVR-mediated photodamage in mice skin. Hairless mice were photoaged for 6 weeks and then treated singly or in combination with LBP and PBM. Histological, immunohistochemistry, and immunofluorescence analyses were used to investigate the levels of c-Fos, c-Jun, MMP-1, -2, and -9, collagen I, III, and FGF2. The combined regimen inhibited UVR-induced skin thickening, decreased the expression of c-Fos and c-Jun, as well as MMP-1, -2, and -9 and concomitantly increased the levels of collagen I, III, and FGF2. The PBM in combination with LBP treatment is a promising strategy for the repair of photodamaged skin, presenting potential clinical application in skin rejuvenation. (AU) | |
| Processo FAPESP: | 13/27021-8 - Efeitos do extrato do fruto de Lycium barbarum L. e do laser vermelho na prevenção e tratamento do fotoenvelhecimento cutâneo em camundongos sem pelos SKH:hr-1 |
| Beneficiário: | Lia Mara Grosso Neves |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 12/25299-6 - Estudos integrados para o controle de formigas cortadeiras |
| Beneficiário: | João Batista Fernandes |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 15/24940-8 - Avaliação da eficácia de mudanças estruturais do [10]-gingerol em combinação com o quimioterápico doxorubicina para o tratamento de câncer de mama: estudos in vitro e in vivo |
| Beneficiário: | Márcia Regina Cominetti |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |