| Texto completo | |
| Autor(es): |
Zamarrenho, Luana Goncalves
;
de Lima, Mikhael Haruo Fernandes
;
Hori, Juliana Issa
;
Lima, Jessica Aparecida
;
Ambrosio, Sergio Ricardo
;
Bastos, Jairo Kenupp
;
De Jong, David
;
Berretta, Andresa Aparecida
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | APPLIED SCIENCES-BASEL; v. 13, n. 10, p. 17-pg., 2023-05-19. |
| Resumo | |
Propolis is known for its immunomodulatory properties. We investigated the effects of three recently developed propolis extract formulations: polar propolis fraction (PPF), soluble propolis dry extract (PSDE), and microencapsulated propolis extract (MPE), and some of their components, on pro- and anti-inflammatory cytokine production in a macrophage model. Bone marrow cell-derived macrophages (BMDM) in cell culture were E. coli lipopolysaccharide (500 ng/mL) stimulated for two hours and subsequently incubated for 20 hours with one of the three propolis extract formulations (1, 10, 25, 50, 100 and 300 mu g/mL) or with isolated propolis components (caffeic acid, p-coumaric acid, artepillin C, or baccharin) (10, 25, 50 and 100 mu g/mL) to determine how they affected secretion of the pro-inflammatory cytokines IL-6 and TNF-alpha, and the anti-inflammatory cytokine, IL-10. PPF increased IL-6 and IL-10 levels. PSDE increased IL-6 and IL-10 at lower concentrations, while at higher concentrations it increased TNF- alpha and decreased IL-10. MPE increased IL-10. Caffeic acid and PPF increased both IL-6 and IL-10. Artepillin C and PSDE decreased IL-10. Baccharin and MPE increased IL-10. Baccharin also decreased IL-6. p-coumaric acid did not affect secretion of these cytokines. Pro- and anti-inflammatory cytokine production by the different propolis extracts differed; however, all three propolis extract formulations have potential as immunomodulatory agents in food supplement and pharmaceutical products. (AU) | |
| Processo FAPESP: | 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro |
| Beneficiário: | Jairo Kenupp Bastos |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |