Reactivity against Sarcocystis neurona and Sarcocystis falcatula-like in horses from Southeastern and Midwestern Brazil

Autor(es)

da Silva, Thiago Merighi Vieira ; De Santi, Mariele ; Goncalves, Luiz Ricardo ; Merino, Marcia Mariza Jusi ; Andre, Marcos Rogerio ; Machado, Rosangela Zacarias

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 32, n. 3, p. 7-pg., 2023-01-01.

Resumo

Equine protozoal myeloencephalitis (EPM) is a neurological disease caused by Sarcocystis neurona. Immunofluorescence antibody tests (IFATs) have been widely used to identify exposure of horses to S. neurona in Brazil. Here we used IFAT to search for IgG antibodies against Sarcocystis falcatula-like (Dal-CG23) and S. neurona (SN138) in sera from 342 horses sampled in Campo Grande, Mato Grosso do Sul state (Midwestern), and Sao Paulo, Sao Paulo state (Southeastern), Brazil. The 1:25 cutoff value was chosen to maximize sensitivity of the test. IgG antibodies against S. neurona were detected in 239 horses (69.88%), whereas IgG antibodies against S. falcatula-like were detected in 177 horses (51.75%). Sera from 132 horses (38.59%) reacted against both isolates. Absence of reactivity was evidenced in 58/342 horses (16.95%). The lower cutoff used, and the presence of opossums infected with S. falcatula-like and Sarcocystis spp. in the regions where the horses were sampled, might justify the high seroprevalence observed here. Owing to the similarity among antigens targeted in immunoassays, reports on S. neurona-seropositive horses in Brazil may also derive from the exposure of horses to other Sarcocystis species. The role of other Sarcocystis species in causing neurological diseases in horses in Brazil remains unclear. (AU)

• Processo FAPESP: 19/08294-0 - Detecção e caracterização molecular de Sarcocystis spp. em Marsupiais (Didelphis spp.) amostrados em Campo Grande, MS e São Paulo, SP, Brasil
• Beneficiário: Mariele de Santi
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 20/06817-2 - Estudo sorológico e molecular acerca de Sarcocystis spp. em equinos e gambás-de-orelha-branca (Didelphis albiventris) na cidade de Campo Grande, MS - Brasil
• Beneficiário: Thiago Merighi Vieira da Silva
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/09915-8 - Detecção e caracterização molecular de Sarcocystis spp. em marsupiais (Didelphis spp.) amostrados em Campo Grande, Mato Grosso do Sul
• Beneficiário: Rosangela Zacarias Machado
• Modalidade de apoio: Auxílio à Pesquisa - Regular