| Texto completo | |
| Autor(es): |
da Silva, Diogo Tiago
;
Alves, Maria Luana
;
Spada, Julio Cesar Pereira
;
Leonel, Joao Augusto Franco
;
Vioti, Geovanna
;
Benassi, Julia Cristina
;
Carregaro, Valeria Maria Lara
;
Alves-Martin, Maria Fernanda
;
Starke-Buzetti, Wilma Aparecida
;
Oliveira, Tricia Maria Ferreira de Sousa
Número total de Autores: 10
|
| Tipo de documento: | Artigo Científico |
| Fonte: | REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 32, n. 2, p. 13-pg., 2023-01-01. |
| Resumo | |
One hundred and sixty-six cats from two animal shelters were subjected to enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence antibody test (IFAT), conventional polymerase chain reaction (cPCR), quantitative PCR (qPCR) and parasitological tests (PA) for the diagnosis of Leishmania spp. Among them, 15% (25/166), 53.6% (89/166), 3.6% (06/166) and 1.8% (03/166) were positive by ELISA, IFAT, both PCRs and PA, respectively. The sequencing of ITS-1 PCR amplicons revealed a 100% match with Leishmania infantum. After the Leishmania spp. survey, 12 cats were selected and divided into two groups for clinical, hematological, and biochemical analysis: six L. infantum positive cats (G1) and six Leishmania spp. negative cats (G2). All the cats were negative for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). A statistical analysis indicated significantly low platelet counts and significant hyperproteinemia associated with hypoalbuminemia in positive cats (p<0.05). Our results suggest that in endemic areas, cats with clinical signs of feline leishmaniosis (such as skin lesions, weight loss and/or enlarged lymph nodes) and that exhibit hematological and biochemical changes, such as low platelet counts and hyperproteinemia with hypoalbuminemia, should be tested for Leishmania spp. infection. (AU) | |
| Processo FAPESP: | 16/08018-4 - Resposta imune humoral e celular de gatos e cavalos infectados por Leishmania spp. |
| Beneficiário: | Trícia Maria Ferreira de Sousa Oliveira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |