| Texto completo | |
| Autor(es): |
Berto, Gabriela L.
;
Mattos, Bruno D.
;
Velasco, Josman
;
Zhao, Bin
;
Segato, Fernando
;
Rojas, Orlando J.
;
Arantes, Valdeir
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | International Journal of Biological Macromolecules; v. 243, p. 9-pg., 2023-06-13. |
| Resumo | |
Enzymatic processing is considered a promising approach for advancing environmentally friendly industrial processes, such as the use of endoglucanase (EG) enzyme in the production of nanocellulose. However, there is ongoing debate regarding the specific properties that make EG pretreatment effective in isolating fibrillated cellulose. To address this issue, we investigated EGs from four glycosyl hydrolase (GH) families (5, 6, 7, and 12) and examined the roles of the three-dimensional structure and catalytic features, with a focus on the presence of a carbohydrate binding module (CBM). Using eucalyptus Kraft wood fibers, we produced cellulose nanofibrils (CNFs) through mild enzymatic pretreatment, followed by disc ultra-refining. Comparing the results with the control (without pretreatment), we observed that GH5 and GH12 enzymes (without CBM) reduced fibrillation energy by approximately 15 %. The most significant energy reduction, 25 and 32 %, was achieved with GH5 and GH6 linked to CBM, respectively. Notably, these CBM-linked EGs improved the rheological properties of CNF suspensions without releasing soluble products. In contrast, GH7-CBM exhibited significant hydrolytic activity, resulting in the release of soluble products, but did not contribute to a reduction in fibrillation energy. This discrepancy can be attributed to the large molecular weight and wide cleft of GH7-CBM, which led to the release of soluble sugars but had little impact on fibrillation. Our findings suggest that the improved fibrillation observed with EG pretreatment is primarily driven by efficient enzyme adsorption on the substrate and modification of the surface viscoelasticity (amorphogenesis), rather than hydrolytic activity or release of products. (AU) | |
| Processo FAPESP: | 15/02862-5 - NANOCEL - desenvolvimento de preparos enzimáticos para o preparo de nanoceluloses por um processo biohídrido |
| Beneficiário: | Valdeir Arantes |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores |
| Processo FAPESP: | 19/22284-7 - Caracterização bioquímica, funcional e aplicação de monoxigenases líticas de polissacarídeos identificadas em estudos de transcriptoma e secretoma de fungos filamentosos cultivados em bagaço de cana |
| Beneficiário: | Fernando Segato |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Regular |
| Processo FAPESP: | 21/07023-2 - Desenvolvimento de uma plataforma tecnologia baseada em enzimas para produção e modulação de nanocelulose: validação para aplicações biomédicas e embalagens ativas |
| Beneficiário: | Valdeir Arantes |
| Modalidade de apoio: | Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2 |
| Processo FAPESP: | 21/06679-1 - Oxidação enzimática do bagaço de cana-de-açúcar II: explorando a interação entre CDHs e LPMOs visando o desenvolvimento de enzimas mais eficientes para inserção em um cell factory geneticamente modificado |
| Beneficiário: | Fernando Segato |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores - Fase 2 |