An Easy-to-Use Histological Technique for Small Biological Samples of Senegalese Sole Larvae

Autor(es)

Santana, Ligia M. B. M. ; Damasceno, Evila Pinheiro ; Loureiro, Susana ; Soares, Amadeu M. V. M. ; Pousao-Ferreira, Pedro ; Abessa, Denis M. S. ; Martins, Roberto ; Pavlaki, Maria D.

• Tipo de documento: Artigo Científico
• Fonte: APPLIED SCIENCES-BASEL; v. 13, n. 4, p. 12-pg., 2023-02-01.

Resumo

Histological techniques are a traditional and essential tool for adequately comprehending cellular morphology and physiology in several scientific fields, including ecotoxicological studies. Although bioassays generally recommend using small test organisms, based on advantages such as low maintenance costs and the possibility of a higher number of replicates, the histological process of such organisms is still challenging due to the difficulty of handling them. The present study proposes an accessible protocol for performing the histology of a biological sample of 8 mm maximum length and using histopathological biomarkers in Senegal sole larvae for ecotoxicological tests. Eight-day old larvae of Solea senegalensis obtained from breeders' natural spawns in cultivation conditions were submitted to histological processing by an adjusted protocol comprising fixation, agarose array pre-embedding, dehydration, paraffin embedding, slides' preparation, and image analysis by light optical microscope coupled to a digital camera. Low-cost and easy-to-use alternatives were applied in the technique execution. The histological steps were detailed, and S. senegalensis morphological features were presented. This can be considered valuable background information for ecotoxicological studies where changes in cell morphology and physiology are expected. (AU)

• Processo FAPESP: 20/03004-0 - Avaliação da bioacumulação e toxicidade de nanomateriais anti-incrustantes inovadores em invertebrados marinhos neotropicais e subtropicais
• Beneficiário: Denis Moledo de Souza Abessa
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 18/07098-0 - Efeito de fármacos marinhos em Peixe Zebra e na linhagem ZFL - emphasis
• Beneficiário: Leticia Veras Costa Lotufo
• Modalidade de apoio: Auxílio à Pesquisa - Regular