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Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation

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Garrigos, Murilo M. ; Oliveira, Fernando A. ; Nucci, Mariana P. ; Mamani, Javier B. ; Dias, Olivia F. M. ; Rego, Gabriel N. A. ; Junqueira, Mara S. ; Costa, Cicero J. S. ; Silva, Lucas R. R. ; Alves, Arielly H. ; Valle, Nicole M. E. ; Marti, Luciana ; Gamarra, Lionel F.
Número total de Autores: 13
Tipo de documento: Artigo Científico
Fonte: PHARMACEUTICS; v. 15, n. 3, p. 18-pg., 2023-03-01.
Resumo

Bone marrow transplantation is a treatment for a variety of hematological and non-hematological diseases. For the transplant success, it is mandatory to have a thriving engraftment of transplanted cells, which directly depends on their homing. The present study proposes an alternative method to evaluate the homing and engraftment of hematopoietic stem cells using bioluminescence imaging and inductively coupled plasma mass spectrometry (ICP-MS) associated with superparamagnetic iron oxide nanoparticles. We have identified an enriched population of hematopoietic stem cells in the bone marrow following the administration of Fluorouracil (5-FU). Lately, the cell labeling with nanoparticles displayed the greatest internalization status when treated with 30 mu g Fe/mL. The quantification by ICP-MS evaluate the stem cells homing by identifying 3.95 +/- 0.37 mu g Fe/mL in the control and 6.61 +/- 0.84 mu g Fe/mL in the bone marrow of transplanted animals. In addition, 2.14 +/- 0.66 mg Fe/g in the spleen of the control group and 2.17 +/- 0.59 mg Fe/g in the spleen of the experimental group was also measured. Moreover, the bioluminescence imaging provided the follow up on the hematopoietic stem cells behavior by monitoring their distribution by the bioluminescence signal. Lastly, the blood count enabled the monitoring of animal hematopoietic reconstitution and ensured the transplantation effectiveness. (AU)

Processo FAPESP: 16/21470-3 - Ação terapêutica das células tronco mesenquimais da medula humana, marcadas com nanopartículas multimodais em ratos diabéticos submetidos a isquemia cerebral focal: estudo dos mecanismos celulares, moleculares e funcionais.
Beneficiário:Lionel Fernel Gamarra Contreras
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/17868-4 - Estudo in vitro da detecção da marcação das células-tronco mesenquimais humanas com nanopartículas magnéticas multimodais com dupla fluorescência mediante as técnicas de ressonância magnética, fluorescência e bioluminescência
Beneficiário:Yolanda Oliveira Pinto
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 19/21070-3 - Avaliação da eficácia terapêutica das CTM marcadas com nanopartículas associada à atividade física no modelo de isquemia focal
Beneficiário:João Victor Matias Ferreira
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica