Autor(es)

Figueiredo, Amanda ; Anholeto, Luis Adriano ; Cola, Diego Faria ; Fantatto, Rafaela Regina ; Gainza, Yousmel Aleman ; dos Santos, Isabella Barbosa ; Vicozzi, Gabriel Pedroso ; Avila, Daiana Silva ; Fraceto, Leonardo Fernandes ; Chagas, Ana Carolina de Souza

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Parasitology; v. 318, p. 11-pg., 2023-04-11.

Resumo

Nanoformulations containing zein nanoparticles (ZN) can promote the stability and protection of molecules with acaricidal activity. The present study sought to develop nanoformulations with ZN associated with cypermethrin (CYPE) + chlorpyrifos (CHLO) + a plant compound (citral, menthol or limonene), characterize them, and verify their efficacy against Rhipicephalus microplus ticks. Additionally, we aimed to assess its safety in nontarget nematodes found in soil at a site subjected to contamination by acaricides. The nanoformulations were char-acterized by dynamic light scattering and nanoparticle tracking analysis. Nanoformulations 1 (ZN+CYPE+-CHLO+citral), 2 (ZN+CYPE+CHLO+menthol), and 3 (ZN+CYPE+CHLO+limonene) were measured for diameter, polydispersion, zeta potential, concentration, and encapsulation efficiency. Nanoformulations 1, 2, and 3 were evaluated in a range from 0.004 to 0.466 mg/mL on R. microplus larvae and caused mortality > 80% at concentrations above 0.029 mg/mL. The commercial acaricide Colosso (R) (CYPE 15 g + CHLO 25 g + citronellal 1 g) was evaluated also from 0.004 to 0.512 mg/mL and resulted in 71.9% larval mortality at 0.064 mg/mL. Formulations 1, 2, and 3 at 0.466 mg/mL showed acaricidal efficacy of 50.2%, 40.5%, and 60.1% on engorged females, respectively, while Colosso (R) at 0.512 mg/mL obtained only 39.4%. The nanoformulations exhibited long residual period of activity and lower toxicity to nontarget nematodes. ZN was able to protect the active compounds against degradation during the storage period. Thus, ZN can be an alternative for the development of new acaricidal formulations using lower concentrations of active compounds. (AU)

• Processo FAPESP: 19/20185-1 - Espilantol e seu potencial antiparasitário e antimicrobiano: estudo dos efeitos em carrapatos de importância médica e veterinária e nos patógenos por eles transmitidos
• Beneficiário: Luís Adriano Anholeto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/16070-1 - Estudo do impacto da infecção por nematóides gastrintestinais no desempenho de Cordeiros Morada Nova para desenvolvimento de método seletivo de controle parasitário
• Beneficiário: Isabella Barbosa dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/13249-8 - Nanotecnologia aplicada à elaboração de carrapaticidas sintéticos associados a isolados vegetais para o controle de Rhipicephalus (b.) microplus
• Beneficiário: Amanda Figueiredo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/21004-5 - Agricultura, micro/nanotecnologia e ambiente: da avaliação dos mecanismos de ação a estudos de transporte e toxicidade
• Beneficiário: Leonardo Fernandes Fraceto
• Modalidade de apoio: Auxílio à Pesquisa - Temático