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Cytotoxicity analysis of vertically aligned multi-walled carbon nanotubes by colorimetric assays

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Autor(es):
Lobo, A. O. ; Corat, M. A. F. ; Antunes, E. F. ; Palma, M. B. S. ; Pacheco-Soares, C. ; Corat, E. J.
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: Synthetic Metals; v. 159, n. 21-22, p. 2-pg., 2009-11-01.
Resumo

A new methodology to evaluate the acute cytotoxicity of vertically aligned multi-walled carbon nanotube (VACNT) scaffolds was presented. A comparison between three different colorimetric assays was performed: (i) lactate dehydrogenase,(ii)2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide, and (iii) neutral red. The results confirmed that a simple removing of VACNT scaffolds from the cell culture before the colorimetric assays, is necessary to obtain a high level of cell viability. (C) 2009 Published by Elsevier B.V. (AU)

Processo FAPESP: 08/11642-5 - Desenvolvimento de estruturas à base de nanotubos de carbono, polímeros biorreabsorvíveis e fibra de carbono para estudos envolvendo células regenerativas e células-tronco hematopoiéticas
Beneficiário:Anderson de Oliveira Lobo
Modalidade de apoio: Bolsas no Brasil - Doutorado