Effects of Lidocaine Infusion during Experimental Endotoxemia in Horses

Autor(es)

Peiro, J. R. ; Barnabe, P. A. ; Cadioli, F. A. ; Cunha, F. Q. ; Lima, V. M. F. ; Mendonca, V. H. ; Santana, A. E. ; Malheiros, E. B. ; Perri, S. H. V. ; Valadao, C. A. A.

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF VETERINARY INTERNAL MEDICINE; v. 24, n. 4, p. 9-pg., 2010-07-01.

Resumo

Background The clinical efficacy of IV infusion of lidocaine for treatment of equine endotoxemia has not been studied. Hypothesis Lidocaine infusion after exposure to lipopolysaccharide (LPS) will inhibit the inflammatory response and have inhibitory effects on the hemodynamic and cytokine responses to endotoxemia. Animals Twelve horses. Methods Two equal groups (n = 6): saline (GI) and lidocaine (GII). In all animals, endotoxin (500 ng/kg body weight [BW]) was injected intraperitoneally over 5 minutes. Twenty minutes later, animals received a bolus of GI or GII (1.3 mg/kg BW) over 5 minutes, followed by a 6-hour continuous rate infusion of GI or GII (0.05 mg/kg BW/min). Treatment efficacy was judged from change in arterial blood pressure, peripheral blood and peritoneal fluid (PF) variables (total and differential cell counts, enzyme activities, and cytokine concentrations), and clinical scores (CS) for behavioral evidence of abdominal pain or discomfort during the study. Results Compared with the control group, horses treated with lidocaine had significantly lower CS and serum and PF tumor necrosis factor-alpha (TNF-alpha) activity. At several time points in both groups, total and differential cell counts, glucose, total protein and fibrinogen concentrations, and alkaline phosphatase, creatine kinase, and TNF-alpha activities were significantly different from baseline values both in peripheral blood and in PF. Conclusions and Clinical Importance Lidocaine significantly decreased severity of CS and inhibited TNF-alpha activity in PF. (AU)

• Processo FAPESP: 02/02448-4 - Mensuração da concentração de óxido nítrico (NO) e da atividade da interleucina-6 (IL-6) no soro e líquido peritoneal de equinos submetidos a endotoxemia experimental seguida da infusão contínua de lidocaína
• Beneficiário: Carlos Augusto Araújo Valadão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/51529-8 - Endotoxemia experimental em equinos: avaliacao da atividade da interleucina-6 (il-6) no soro e liquido peritoneal pelo metodo de elisa, antes e apos a infusao continua de licocaina.
• Beneficiário: Victor Hugo Mendonça
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica