| Texto completo | |
| Autor(es): |
Colussi, Francieli
;
Textor, Larissa C.
;
Serpa, Viviane
;
Maeda, Roberto Nobuyuki
;
Pereira, Nei, Jr.
;
Polikarpov, Igor
Número total de Autores: 6
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 66, p. 4-pg., 2010-09-01. |
| Resumo | |
The filamentous fungus Trichoderma harzianum has a considerable cellulolytic activity that is mediated by a complex of enzymes which are essential for the hydrolysis of microcrystalline cellulose. These enzymes were produced by the induction of T. harzianum with microcrystalline cellulose (Avicel) under submerged fermentation in a bioreactor. The catalytic core domain (CCD) of cellobiohydrolase I (CBHI) was purified from the extracellular extracts and submitted to robotic crystallization. Diffraction-quality CBHI CCD crystals were grown and an X-ray diffraction data set was collected under cryogenic conditions using a synchrotron-radiation source. (AU) | |
| Processo FAPESP: | 09/05349-6 - Expressão e purificação da celobiohidrolase I do fungo filamentoso Trichoderma harzianum |
| Beneficiário: | Bruno Luan Soares Paula de Mello |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 07/08706-9 - ESTUDOS MOLECULARES E ESTRUTURAIS DE ENZIMAS DO COMPLEXO CELULOLÍTICO DO FUNGO Trichoderma harzianum COM POTENCIAL NA TRANSFORMAÇÃO ENZIMÁTICA DA BIOMASSA |
| Beneficiário: | Viviane Isabel Serpa |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |