Evaluation of caries-affected dentin with optical coherence tomography

Autor(es)

de Azevedo, Cynthia Soares ; Espejo Trung, Luciana Cardoso ; Lorenzetti Simionato, Maria Regina ; de Freitas, Anderson Zanardi ; Matos, Adriana Bona

• Tipo de documento: Artigo Científico
• Fonte: BRAZILIAN ORAL RESEARCH; v. 25, n. 5, p. 7-pg., 2011-09-01.

Resumo

The purpose of this study was to evaluate the degree of demineralization of artificially induced caries-affected human dentin by an in vitro microbiological method. The occlusal surfaces of 6 human molar teeth were abraded until a flat surface was obtained, and the enamel was removed to expose the occlusal dentin surface. These teeth were sectioned in 12 halves in the vestibular-lingual direction and divided into 3 groups according to the period length of the microbiological essay (n = 4): G1, 7 days; G2, 14 days; and G3, 21 days. The surfaces of all specimens were protected by an acid-resistant nail varnish, except for a window where the caries lesion was induced by a Streptoccocus mutans biofilm in a batch-culture model supplemented with 5% sucrose. The specimens were then analyzed by optical coherence tomography (OCT) with a super-luminescent light diode (lambda = 930 nm) with 6.0-mu m lateral and longitudinal resolution (in the air). Qualitative and quantitative results (images and average dentin demineralization, respectively) were obtained. The mean demineralization depths were (mu m) 235 +/- 31.4, 279 +/- 14, and 271 +/- 8.3 in groups 1, 2, and 3, respectively. In addition, no significant change was observed in the lesion mean depth from 7 days of cariogenic challenge on. In conclusion, OCT was shown to be an efficient and non-invasive method to detect the depths of lesions caused by demineralization. Further, a seven-day demineralization time was considered sufficient for caries-affected dentin to be obtained. (AU)

• Processo FAPESP: 09/07709-0 - Estudo de adesão em dentina remanescente após remoção de tecido cariado com lasers de alta intensidade
• Beneficiário: Cynthia Soares de Azevedo
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica