Endophytic Streptomyces tubercidicus isolated from Solanun lycocarpum St Hill: A sheriff secondary metabolites producer

Autor(es)

Ratti, R. P. ; Granato, A. C. ; Hokka, C. O. ; Badino-Junior, A. C. ; Lima, L. C. P. S. ; Paiva de Sousa, C. ; MendezVilas, A

• Tipo de documento: Artigo Científico
• Fonte: MICROBES IN APPLIED RESEARCH: CURRENT ADVANCES AND CHALLENGES; v. N/A, p. 5-pg., 2012-01-01.

Resumo

The bioactivity and purification of compounds produced by endophytic S. tubercidicus isolated from Solanum lycocarpum St. Hill, a typical Brazilian tropical savannah tree, was achieved. S. tubercidicus presented bioactivity against S. aureus (ATCC 29213), E. coli (ATCC 25922) and C. albicans (ATCC 10231) with 35 mm, 40 mm, and 19 mm antibiosis hales, respectively, but failed to inhibit P. aeruginosa (ATCC 27853). The growing kinetics was evaluated during 21 days and the major quantification detection was 7.88 log CFU.mL(-1), observed at the second day of incubation. The fresh crude extract showed a maximum antimicrobial potential of 200 AU.mL(-1) against E. coli and S. aureus. Separation of crude extract of S. tubercidicus on Sephadex LH-20 gel methanol revealed two compounds presenting bioactivity against S. aureus and E. coli. Analysis under UV illumination showed three distinct compounds, two of them were less polar (254 nm) and one was more polar (365 nm). All these findings can contribute to the characterization of new and useful antimicrobial substances with various applications. (AU)

• Processo FAPESP: 05/55079-4 - Produção e purificação de ácido clavulânico, cefamicina C e outros metabolitos bioativos de Streptomyces
• Beneficiário: Carlos Osamu Hokka
• Modalidade de apoio: Auxílio à Pesquisa - Temático