| Texto completo | |
| Autor(es): |
Rosseto, Flavio R.
;
Puhl, Ana C.
;
Andrade, Maxuel O.
;
Polikarpov, Igor
Número total de Autores: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS; v. 69, p. 4-pg., 2013-02-01. |
| Resumo | |
Cellulases, such as endoglucanases, exoglucanases and beta-glucosidases, are important enzymes used in the process of enzymatic hydrolysis of plant biomass. The bacteria Xanthomonas campestris pv. campestris expresses a large number of hydrolases and the major endoglucanase (XccEG), a member of glycoside hydrolase family 5 (GH5), is the most strongly secreted extracellularly. In this work, the native XccEG was purified from the extracellular extract and crystallization assays were performed on its catalytic domain. A complete data set was collected on an in-house X-ray source. The crystal diffracted to 2.7 angstrom resolution and belonged to space group C2, with unit-cell parameters a = 174.66, b = 141.53, c = 108.00 angstrom, beta = 110.49 degrees. The Matthews coefficient suggests a solvent content of 70.1% and the presence of four protein subunits in the asymmetric unit. (AU) | |
| Processo FAPESP: | 10/52362-5 - Targeted analysis of microbial lignocellulolytic secretomes: a new approach to enzyme discovery |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 09/05349-6 - Expressão e Purificação da Celobiohidrolase I do Fungo filamentoso Trichoderma harzianum |
| Beneficiário: | Bruno Luan Soares Paula de Mello |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 08/56255-9 - Structure and function of enzymes and auxiliary proteins from Trichoderma, active in cell-wall hydrolysis |
| Beneficiário: | Igor Polikarpov |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOEN - Temático |
| Processo FAPESP: | 07/08706-9 - ESTUDOS MOLECULARES E ESTRUTURAIS DE ENZIMAS DO COMPLEXO CELULOLÍTICO DO FUNGO Trichoderma harzianum COM POTENCIAL NA TRANSFORMAÇÃO ENZIMÁTICA DA BIOMASSA |
| Beneficiário: | Viviane Isabel Serpa |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |