Usefulness of serological ELISA assay for Taenia saginata to detect naturally infected bovines

Autor(es)

Paulan, Silvana de Cassia ; Hernandes Gonzales, Rutilia Marisela ; Peralta, Laura Adalid ; Vicentini-Oliveira, Josy Campanha ; Biondi, Germano Francisco ; Conde, Edda Sciuto ; Evans Parkhouse, Robert Michael ; Nunes, Caris Maroni

• Tipo de documento: Artigo Científico
• Fonte: REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA; v. 22, n. 2, p. 6-pg., 2013-04-01.

Resumo

Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T solium vesicular fluid and T saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ((X) over bar + 2DP for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis. (AU)

• Processo FAPESP: 09/12719-4 - Desenvolvimento de teste elisa sanduíche para cisticercose bovina
• Beneficiário: Silvana de Cássia Paulan
• Modalidade de apoio: Bolsas no Brasil - Mestrado