Anthocyanin Incorporated Dental Copolymer: Bacterial Growth Inhibition, Mechanical Properties, and Compound Release Rates and Stability by H-1 NMR

Autor(es)

Hrynash, Halyna ; Pilly, Vinay Kumar ; Mankovskaia, Alexandra ; Xiong, Yaoyang ; Nogueira Filho, Getulio ; Bresciani, Eduardo ; Levesque, Celine Marie ; Prakki, Anuradha

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF DENTISTRY; v. 2014, p. 6-pg., 2014-01-01.

Resumo

Objective. To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY; Vaccinium macrocarpon). Methods. Resin samples were prepared (Bis-GMA/TEGDMA at 70/30mol%) and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX), except for the control group. Samples were individually immersed in a bacterial culture (Streptococcus mutans) for 24 h. Cell viability (n = 3) was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS) and elastic modulus (E) were tested on a universal testing machine (n = 8). Compound release and chemical stability were evaluated by UV spectrophotometry and H-1 NMR (n = 3). Data were analyzed by one-way ANOVA and Tukey's test (alpha = 0.05). Results. Both compounds inhibited S. mutans growth, with CHX being most effective (P < 0.05). Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties (P < 0.05). The 24 h compound release rates were ACY: 1.33 mu g/mL and CHX: 1.92 mu g/mL. H-1 NMR spectra suggests that both compounds remained stable after being released in water. Conclusion. The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials. (AU)

• Processo FAPESP: 12/19223-7 - Influência do tratamento clareador e envelhecimento induzido sobre o brilho, a cor, a rugosidade e a microdureza superficial de resinas compostas
• Beneficiário: Eduardo Bresciani
• Modalidade de apoio: Auxílio à Pesquisa - Regular