Evaluation of carbon nanotubes network toxicity in zebrafish (danio rerio) model

Autor(es)

de Souza Filho, Jose ; Matsubara, Elaine Y. ; Franchi, Leonardo Pereira ; Martins, Igor Pinheiro ; Ramires Rivera, Luis Miguel ; Rosolen, Jose Mauricio ; Grisolia, Cesar Koppe

• Tipo de documento: Artigo Científico
• Fonte: Environmental Research; v. 134, p. 8-pg., 2014-10-01.

Resumo

This is a detailed in vivo study of the biological response to carbon nanotubes network as probed by the zebrafish model. First, we prepared pristine carbon nanotubes (CNTs) by methanol chemical vapor deposition in the presence of Mn and Co as catalysts, followed by purification in acid, which furnished curved tubes with diameters lying between 10 and 130 nm. The CNT network consisted of pristine CNTs dispersed in water in the presence of a surfactant. The CNT network pellets corresponded to agglomerated multi-walled CNTs with an average diameter of about 500 nm. Although the same pristine CNTs had been previously found to exert genotoxic effects in vitro, here we verified that the CNT network was not genotoxic in vivo. Indeed, Raman spectroscopy and microscopy conducted in the intestine of the zebrafish revealed complete clearance of the CNT network as well as minimal disturbances, such as aneurysms, hyperemia, and reversible inflammatory focus in the zebrafish gills. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 10/07681-5 - Desenvolvimento e estudo de materiais micro/nanoestruturados de interesse para baterias de íons de lítio e capacitores
• Beneficiário: Jose Mauricio Rosolen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/10863-8 - Efeitos citotóxicos e genotóxicos de nanopartículas em linhagens celulares in vitro.
• Beneficiário: Leonardo Pereira Franchi
• Modalidade de apoio: Bolsas no Brasil - Doutorado