Sperm characteristics of Steindachneridion parahybae (Steindachner, 1877) throughout 112h of storage at four temperatures

Autor(es)

Sanches, E. A. ; Okawara, R. Y. ; Caneppele, D. ; Toledo, C. P. R. ; Bombardelli, R. A. ; Romagosa, E.

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Ichthyology; v. 31, p. 10-pg., 2015-06-01.

Resumo

The objective of this study was to assess the effect of storage temperature on the fresh sperm quality of Steindachneridion parahybae over 112h of storage. Semen collected from five males was tested in a 12x4 factorial experimental design using the sperm storage times of: 0 (control), 1, 2, 4, 8, 16, 24, 36, 48, 64, 88, and 112h after collection. Temperatures tested were (mean +/- SD): 5.2 +/- 3.4, 12.9 +/- 1.6, 24.4 +/- 1.7 and 34.9 +/- 1.1 degrees C. Sperm parameters were motility rate (MOT), curvilinear velocity (VCL), average path velocity (VAP), straight line velocity (VSL), straightness (STR), sperm normality (NOR) and survival rate (SUR). The parameters VCL, VAP and VSL were grouped by principal component analysis to create the sperm velocity (VS). The parameters assessed exhibited effects (P<0.05) of the exposure time and temperature. Interactive effects between temperature and time (P<0.05) were observed for STR, NOR and SUR, and quadratic effects for factors MOT and VS (P<0.05). Pearson's linear correlation was verified between the parameters studied (P<0.05), indicating MOT, VCL and STR as good indicators of sperm quality. Chilling at temperatures around 5 degrees C and heating at around 35 degrees C were harmful to S. parahybae spermatozoa. It is suggested that short-term (up to 8h) semen storage - if necessary - should be within a range of 15-25 degrees C. (AU)

• Processo FAPESP: 09/18609-6 - QUALIDADE DOS GAMETAS E FERTILIZAÇÃO ARTIFICIAL DE SURUBIM-DO-PARAÍBA, Steindachneridion parahybae
• Beneficiário: Eduardo Antônio Sanches
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/02818-5 - Gametas e fertilização artificial de Surubim-do-Paraíba, Steindachneridion parahybae, espécie ameaçada de extinção
• Beneficiário: Elizabeth Romagosa
• Modalidade de apoio: Auxílio à Pesquisa - Regular