Mycobacterium saopaulense sp nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae-Mycobacterium abscessus group

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Nogueira, Christiane Lourenco ; Whipps, Christopher M. ; Matsumoto, Cristianne Kayoko ; Chimara, Erica ; Droz, Sara ; Tortoli, Enrico ; de Freitas, Denise ; Cnockaert, Margo ; Palomino, Juan Carlos ; Martin, Anandi ; Vandamme, Peter ; Leao, Sylvia Cardoso

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY; v. 65, p. 7-pg., 2015-12-01.

Resumo

Five isolates of non-pigmented, rapidly growing mycobacteria were isolated from three patients and, in an earlier study, from zebrafish. Phenotypic and molecular tests confirmed that these isolates belong to the Mycobacterium chelonae-Mycobacterium abscessus group, but they could not be confidently assigned to any known species of this group. Phenotypic analysis and biochemical tests were not helpful for distinguishing these isolates from other members of the M. chelonae-M. abscessus group. The isolates presented higher drug resistance in comparison with other members of the group, showing susceptibility only to clarithromycin. The five isolates showed a unique PCR restriction analysis pattern of the hsp65 gene, 100 % similarity in 16S rRNA gene and hsp65 sequences and 1-2 nt differences in rpoB and internal transcribed spacer (ITS) sequences. Phylogenetic analysis of a concatenated dataset including 16S rRNA gene, hsp65, and rpoB sequences from type strains of more closely related species placed the five isolates together, as a distinct lineage from previously described species, suggesting a sister relationship to a group consisting of M. chelonae, Mycobacterium salmoniphilum, Mycobacterium franklinii and Mycobacterium immunogenum. DNA-DNA hybridization values >70 % confirmed that the five isolates belong to the same species, while values <70 % between one of the isolates and the type strains of M. chelonae and M. abscessus confirmed that the isolates belong to a distinct species. The polyphasic characterization of these isolates, supported by DNA-DNA hybridization results, demonstrated that they share characteristics with M. chelonae-M. abscessus members, but constitute a different species, for which the name Mycobacterium saopaulense sp. nov. is proposed. The type strain is EPM 10906(T) (=CCUG 66554(T)=LMG 28586(T)=INCOS 0733(T)). (AU)

• Processo FAPESP: 12/13763-0 - Caracterização taxonômica de micobactérias do grupo Mycobacterium chelonae-M. abscessus sem dentificação de espécie conclusiva
• Beneficiário: Christiane Lourenço Nogueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/18326-4 - Micobactérias e seus elementos extracromossômicos: caracterização molecular e aplicações biotecnológicas
• Beneficiário: Sylvia Luisa Pincherle Cardoso Leão
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/16018-6 - Sequenciamento e anotação do genoma da cepa epm 10906, uma nova espécie de micobactéria
• Beneficiário: Cristianne Kayoko Matsumoto
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado