Autor(es)

Andrioli, Luiz Paulo ; dos Santos, Wesley Silva ; Aguiar, Francisco dos Santos ; Digiampietri, Luciano Antonio

• Tipo de documento: Artigo Científico
• Fonte: MECHANISMS OF DEVELOPMENT; v. 144, p. 7-pg., 2017-04-01.

Resumo

We investigated the hypothesis that several transcriptional repressors are necessary to set the boundaries of anterior pair-rule stripes in Drosophila. Specifically, we tested whether Tailless (Tll) is part of a repression mechanism that correctly sets the anterior boundaries of hairy 1 (h 1) and even-skipped 1 (eve 1) stripes. Single mutant tll embryos displayed subtle deviations from the normal positions of h 1 and eve 1 stripes. Moreover, we observed stronger stripe deviations in embryos lacking both Tll and Sloppy-paired 1 (Slp 1), a common repressor for anterior pair-rule stripes. Using h 1 and eve 1 reporter constructs in the genetic assays, we provided further evidence that interference with normal mechanisms of stripe expression is mediated by Tll repression. Indeed, Tll represses both h 1 and eve 1 reporter stripes when misexpressed. Investigating the expression of other anterior gap genes in different genetic backgrounds and in the misexpression assays strengthened Tll direct repression in the regulation of h 1 and eve 1. Our results are consistent with tll being a newly-identified component of a combinatorial network of repressor genes that control pair-rule stripe formation in the anterior blastoderm of Drosophila. (C) 2016 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 11/00096-2 - Investigações da cascata de segmentação na região anterior do embrião de Drosophila melanogaster e do sciarídeo Trichosia pubescens
• Beneficiário: Luiz Paulo Moura Andrioli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/14318-5 - Interações gênicas na região anterior da blastoderme sincicial de Drosophila melanogaster e do sciarídeo Trichosia pubescens
• Beneficiário: Luiz Paulo Moura Andrioli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/12147-4 - Regulação dos genes de segmentação expressos na cabeça no início do desenvolvimento de Drosophila melanogaster
• Beneficiário: Luiz Paulo Moura Andrioli
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores