Surface plasmon resonance biosensor for enzymatic detection of small analytes

Autor(es)

Miyazaki, Celina Massumi ; Shimizu, Flavio Makoto ; Mejia-Salazar, J. R. ; Oliveira, Osvaldo N., Jr. ; Ferreira, Marystela

• Tipo de documento: Artigo Científico
• Fonte: Nanotechnology; v. 28, n. 14, p. 6-pg., 2017-04-07.

Resumo

Surface plasmon resonance (SPR) biosensing is based on the detection of small changes in the refractive index on a gold surface modified with molecular recognition materials, thus being mostly limited to detecting large molecules. In this paper, we report on a SPR biosensing platform suitable to detect small molecules by making use of the mediator-type enzyme microperoxidase-11 (MP11) in layer-by-layer films. By depositing a top layer of glucose oxidase or uricase, we were able to detect glucose or uric acid with limits of detection of 3.4 and 0.27 mu mol l(-1), respectively. Measurable SPR signals could be achieved because of the changes in polarizability of MP11, as it is oxidized upon interaction with the analyte. Confirmation of this hypothesis was obtained with finite difference time domain simulations, which also allowed us to discard the possible effects from film roughness changes observed in atomic force microscopy images. The main advantage of this mediator-type enzyme approach is in the simplicity of the experimental method that does not require an external potential, unlike similar approaches for SPR biosensing of small molecules. The detection limits reported here were achieved without optimizing the film architecture, and therefore the performance can in principle be further enhanced, while the proposed SPR platform may be extended to any system where hydrogen peroxide is generated in enzymatic reactions. (AU)

• Processo FAPESP: 12/15543-7 - Biossensores para detectar Escherichia coli usando o conceito de línguas eletrônicas
• Beneficiário: Flavio Makoto Shimizu
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/15093-7 - Estudos de sistemas nanoestruturados por ressonância plasmônica de superfície (SPR): aplicações em biossensoriamento
• Beneficiário: Celina Massumi Miyazaki
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/12311-9 - Efeito transversal magneto-óptico Kerr para o desenho e desenvolvimento de biosessores
• Beneficiário: Jorge Ricardo Mejía Salazar
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/17519-1 - Imobilização de nanopartículas metálicas em filmes ultrafinos visando ao biossensoriamento
• Beneficiário: Marystela Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático