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Entree


Characterization of teratogenic potential and gene expression in canine and feline amniotic membrane-derived stem cells

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Autor(es):
Cardoso, M. T. ; Pinheiro, A. O. ; Vidane, A. S. ; Casals, J. B. ; de Oliveira, V. C. ; Goncalves, N. J. N. ; Martins, D. S. ; Ambrosio, C. E.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 52, p. 7-pg., 2017-04-01.
Resumo

Contents The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane-derived stem cells (AMSCs). In contrast, tumour formation was observed when canine AMSCs were utilized. These findings suggested that feline and human, but not canine, AMSCs are suitable for cell transplantation trials. This study aimed to further evaluate the feasibility of utilizing canine AMSCs for transplantation purposes as well as for felines. We tested teratoma formation following cell injection into BALB/c nude mice and then assessed expression of haematopoietic, mesenchymal, tumorigenic, pluripotency and cellular regulation markers using flow cytometry and qPCR. The use of canine AMSCs did not result in macroscopic tumour formation as determined 60days after transplantation. The immunophenotypic characterization by flow cytometry revealed expression of mesenchymal markers (CD73 and CD90) and expression of the pluripotent marker OCT4 and SOX2. Quantitative PCR analysis revealed that there were no differences in the patterns of gene expression (CD34, CD73, OCT4, CD30 and P53) between canine and feline AMSCs, with the exception of the expression of SOX2 and CD90. (AU)

Processo FAPESP: 13/09392-9 - Produção de células-tronco de pluripotência induzida como modelo de estudo em coelhos
Beneficiário:Carlos Eduardo Ambrósio
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/23850-0 - Comparação molecular entre células-tronco mesenquimais de membrana amniótica canina e felina
Beneficiário:Mariana Trés Cardoso
Modalidade de apoio: Bolsas no Brasil - Mestrado